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Masamine Takanosu, Tanya C. Boyd, Magali Le Goff, Stephen P. Henry, Youwen Zhang, Paul N. Bishop, Richard Mayne; Structure, Chromosomal Location, and Tissue-Specific Expression of the Mouse Opticin Gene. Invest. Ophthalmol. Vis. Sci. 2001;42(10):2202-2210.
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purpose. To determine the structure, location, and tissue-specific expression of
the mouse opticin gene (Optc) and to compare
expression in the eye with that of Prelp, collagen II,
and collagen IX.
methods. Expressed sequence tags (ESTs) to mouse opticin were identified and the
full-length sequence obtained after PCR reactions using a
15-day-postconception (dpc) whole-mouse embryo cDNA library. The mouse
chromosomal localization of Optc was determined by
radiation hybrid mapping and its genomic structure determined using an Optc-containing BAC clone. Tissue-specific expression of
opticin, PRELP, collagen II, and collagen IX mRNAs was investigated by
in situ hybridization and by dot blot hybridization for opticin.
results. The Optc gene was localized to mouse chromosome 1 at
74.3 cM and consisted of seven exons spanning 10 kb. The Optc gene was less than 4 kb from the Prelp gene. In situ hybridization localized opticin mRNA
exclusively to the presumptive ciliary body during development and to
the nonpigmented ciliary epithelium of the adult mouse eye. Expression
of Prelp was also detected in the nonpigmented ciliary epithelium of
the adult eye. However, expression of collagen types II and IX was
detected largely in the developing mouse eye, with type IX expression
confined primarily to the presumptive ciliary body.
conclusions. The Optc, Prelp, and fibromodulin
(Fmod) genes form a cluster on mouse chromosome 1.
Opticin may represent a marker for ciliary body differentiation.
Continued expression of opticin in the adult mouse eye suggests
functions other than that of putative regulator of vitreous collagen
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