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Douglas A. Jabs, Robert A. Prendergast, Eva M. Rorer, Alan P. Hudson, Judith A. Whittum-Hudson; Cytokines in Autoimmune Lacrimal Gland Disease in MRL/MpJ Mice. Invest. Ophthalmol. Vis. Sci. 2001;42(11):2567-2571.
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purpose. MRL/MpJ-+/+ (MRL/+) and MRL/MpJ-lpr/lpr (MRL/lpr) mice show spontaneous development of a T-cell–driven
lacrimal gland inflammation that is a model for Sjögren syndrome.
The lacrimal gland lesions in these mice were evaluated by quantitative
RT-PCR for selected cytokine mRNA for the relative contributions of
T-helper (Th)1 versus Th2 immune responses and by RT-PCR and
immunohistochemistry for the contribution of the interleukin
(IL)-2/IL-2 receptor (IL-2R) autocrine pathway.
methods. RNA was isolated from lacrimal glands of MRL/+ mice ages 1 to 9 months
and from MRL/lpr mice ages 1 through 5 months, and competitive RT-PCR
was used to quantify mRNA for the cytokines IL-2, -4, -10, and -12 and
interferon (IFN)-γ. Frozen sections of lacrimal glands from MRL/+ and
MRL/lpr mice ages 2 through 5 months were stained for the IL-2R.
results. IL-2 and -12 mRNA transcripts were below the limit of detection
(<10−3 fg/pg hypoxanthine phosphoribosyl transferase
gene; HPRT) in both MRL/+ and MRL/lpr mice of all ages.
When detectable, IFN-γ transcripts were present in low amounts and
were below the limit of detection in most samples. IL-4 transcripts
were present in 100- to 1000-fold greater amounts than IFN-γ
transcripts. IL-10 transcripts were detectable in both MRL/+ and
MRL/lpr mice. IL-2R typically was detected on less than 10% of
lymphocytes infiltrating lacrimal gland lesions in both substrains.
conclusions. On the basis of RT-PCR for cytokine mRNA, autoimmune lacrimal gland
lesions in MRL/+ and MRL/lpr mice appear to be largely Th2-mediated.
There does not appear to be a direct role for the IL-2/IL-2R autocrine
pathway within the microenvironment of the lacrimal
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