September 1973
Volume 12, Issue 9
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Articles  |   September 1973
Absolute Levels of Some Free Ammo Acids in Normal and Biologically Fractionated Retinas
Author Affiliations
  • ADOLPH I. COHEN
    Research funded by Grant EY-00258-10 of the National Institutes of Health. Recipient of Career Development Award EY-03170-09 of the National Institutes of Health.
  • MICHAEL McDANIEL
    Research funded by Grant NS-05221 of the National Institutes of Health and by Grant BC-4 of the American Cancer Society
  • HARRY ORR
    Trainee under Grant NS-05613-05 of the National Institutes of Health
Investigative Ophthalmology & Visual Science September 1973, Vol.12, 686-693. doi:
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      ADOLPH I. COHEN, MICHAEL McDANIEL, HARRY ORR; Absolute Levels of Some Free Ammo Acids in Normal and Biologically Fractionated Retinas. Invest. Ophthalmol. Vis. Sci. 1973;12(9):686-693.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Using isolated (1) normal retinas, (2) receplorless retinas, and (3) retinas with markedly reduced inner layers, respectively, derived from 90-day-old pigmented mice which were normal, or possessed of a genetic receptor dystrophy, or treated postnatally with monosodium glutamate, extracts were assayed with an amino acid analyzer and/or by fluorometric ultramicro techniques. In millimoles per kilogram protein, the glycine, ananine, and GABA levels were, respectively, 17.0, 4.7, and 18.7 in (1), 38.0, 8.7, and 26.0 in (2), and 9.0, 2.9, and 6.0 in (3). These data suggest a relative concentration of these amino acids in the inner retina with glycine and GABA levels in (2) matching or exceeding published values for any central nervous system region. Taurine, however, had concentrations of 410, 150, and 500 in (1), (2), and (3), respectively, thus suggesting high levels everywhere and a relative concentration in the outer retina. In some groups, glutamate, GABA glutamine, or aspartate levels were lower in receptor-containing retinas from dark-adapted as compared to light-adapted animals. Glutamate concentrations were similar in (1), (2), and (3) and aspartate and glutamine plus serine somewhat elevated in (2). Relative neurophysiologic inactivity and/or the different lesion mechanisms in the two abnormal retinas must affect these data. However, when large concentration differences between (1) and (2) and between (1) and (3) are in opposing directions, true distribution differences in (1) are suggested. The data may bear on amino acids as possible neural transmitters and/or on local metabolic specializations.

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