Tissue Culture Study of Human Retinoblastoma

Abstract

Cells derived from four human retinoblastomas were maintained in culture for periods ranging from 2 to 24 months. The tumors grew in vitro in a biphasic manner with an undifferentiated stroma attached to the surface of the tissue culture flask and clusters of well-differentiated retinoblastoma cells growing in suspension. The cells in suspension continued to produce Flexner-Wintersteiner rosettes and on electron microscopy contained microtubules.