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HARRY L. S. KNOPF, DINAH M. BERTRAN, ALBERT Z. KAPIKIAN; Demonstration and Characterization of Antibody in Tears Following Intranasal Vaccination with Inactivated Type 13 Rhinovirus: A Preliminary Report. Invest. Ophthalmol. Vis. Sci. 1970;9(10):727-734.
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Tears were collected with Schirmer strips from volunteers following intranasal instillation of saline or inactivated type 13 rhinovirus (RV13) vaccine. An additional group was challenged intranasally with live RV13. Analysis of the secretions to as made to determine (1) if antiviral activity was present after introduction of antigen at a distant site and (2) the type of antibody responsible for such activity. Methods employed for this analysis were sucrose gradient centrifugation, immunoelectrophoresis, and tissue culture neutralization. The major immunoglobulins in the tears were 11S immuno globulin A (IgA) and 7S immuno globulin G (IgG). Neurtalizing activity was found primarily in the US region of gradients of pooled tear samples; this was associated with IgA. In samples collected from individual challenged volunteers, there was activity associated with IgG as well as IgA. The mechanism responsible for the appearance of specific secretory immuno globulin in the tears following intranasal instillation of an inactivated virus vaccine is not understood.
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