November 1970
Volume 9, Issue 11
Articles  |   November 1970
Protein Release During Aqueous Withdrawal in Rabbits
Author Affiliations
    Bishop Eye Research Center, Pacific Northwest Research Foundation, 1102 Columbia St., Seattle, Wash.
    Bishop Eye Research Center, Pacific Northwest Research Foundation, 1102 Columbia St., Seattle, Wash.
Investigative Ophthalmology & Visual Science November 1970, Vol.9, 865-872. doi:
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      JERROL R. NEUPERT, CARTERET LAWRENCE; Protein Release During Aqueous Withdrawal in Rabbits. Invest. Ophthalmol. Vis. Sci. 1970;9(11):865-872.

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      © ARVO (1962-2015); The Authors (2016-present)

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Aqueous was withdrawn from the anterior chamber of one-year-old male rabbits for one-half hour while intraocular pressure and systemic blood pressure were monitored. Following withdrawal, a sample from each rabbit eye was analyzed for total protein, and another was concentrated by dialysis, then fractionated by cellulose acetate electrophoresis. Albumin/globulin (A/G) ratios were calculated for each sample and correlated with total protein content; aqueous protein concentration was correlated with steady-state intraocular pressure. The A/G ratio of primary aqueous was significantly above that of serum. Only a slight increase in total protein concentration (to 400 mg. per cent) after withdrawal of aqueous caused a precipitous drop in A/G ratio levels equal to or below serum levels. The decrease in A/G ratio of secondary aqueous resulted from a decrease in the per cent of albumin and an increase in the per cent of α- and γ-globulins as aqueous protein concentration increased. The per cent of (β-globulin remained unchanged. Correlation of aqueous protein concentration with final steady-state intraocular pressure suggested a threshold phenomenon for protein release in the rabbit eye when intraocidar pressure approached or fell below expected levels of episcleral venous pressure.


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