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D. W. PLOTH, C. A. HOGBEN; Ion Transport by the Isolated Frog Cornea. Invest. Ophthalmol. Vis. Sci. 1967;6(4):340-347.
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The in vitro preparation of the frog cornea, R. catesbeiana, bathed by modified bicarbonate inger's solution and ivith 0.5 cm. of hydrostatic pressure difference at the aqueous side, developed a spontaneous transcorneal potential difference of approximately -22 mv. with the tear side negative relative to the aqueous. The short circuit current required to bring the potential difference to zero was 410 nEq. cm-2 (11 µa cm-2). The resistance was 2 KΩ cm-2 Simultaneous 22Na and 36Cl unidirectional fluxes were measured with the isolated cornea short circuited. The mean unidirectional sodium fluxes were approximately equal and no significant net transport was demonstrated. The difference between the mean unidirectional chloride fluxes was a net transport of 480 nEq. cm-2 hour-1 from the aqueous to the tear side. The difference ivas statistically indistinguishable from the short circuit current. These findings are in agreement with previous studies of the isolated frog cornea. Further analysis of the unidirectional fluxes of chloride as reversible and irreversible processes relative to the membrane conductance revealed that the chloride fluxes may have a significant component of exchange diffusion.
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