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Abstract
Small Sephadex G-200 columns were employed to purify milligram quantities of DNA extracted from dogfish cornea epithelium with sodium dodecyl sulfate, and studies of the composition and physical properties were made. 3H thymidine and 125I deoxyuridine were incorporated into epithelial DNA under simple in vitro circumstances. The incorporation of thymidine depended upon the temperature and oxygen tension of the incubation medium.