Nucleic Acid Metabolism in the Lens I. Isolation And Composition Of RNA

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Abstract

Ribose nucleic acids of rat and dogfish lenses were extracted by the Duponol method. The nucleotide composition of albuminoid, ribosomal, and soluble RNA ivas determined by alkaline hydrolysis of the various RNA fractions followed by paper chromatography. The ultraviolet absorbing spots were eluted from the chromatograms with HCl and the resulting solutions were subjected to spectral analyses and microorcinol determinations. The results of these determinations, including ultraviolet absorption spectra of the individual nucleotides, are presented. The nucleotide composition of lens RNA is similar to that of other organs (liver and kidney) which have been reported.