Rabbit polyclonal anti-survivin antibody (ab469) and anti-p63 antibody (ab53039) were purchased from Abcam Biotechnology (Cambridge, UK). The antibodies for phosphorylated survivin (Tr43), p63 (TAp63 and deltaNp63), p57, p21, and lamin B (sc-23758, sc-25268, sc-1040, sc-756, sc-6216) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Beta-actin antibody (A-3854) was from Sigma-Aldrich Corp. (St. Louis, MO, USA). Horse radish peroxidase (HRP)–conjugated goat anti-rabbit IgG antibody (170-6515) was from Bio-Rad (Hercules, CA, USA). Fluorescein isothiocyanate–conjugated swine anti-rabbit IgG antibody (F0205) was from Dako Biotechnology (Glostrup, Denmark). Anti-rabbit Isotype control IgG (08-6199) was from Invitrogen (Carlsbad, CA, USA).
Reverse Transcription System was from Promega (Madison, WI, USA). SYBR Premix Ex Taq (Tli RNaseH Plus) was purchased from TaKaRa (Shiga, Japan). Mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) was obtained from Vector Laboratories (Burlingame, CA, USA). Two-step histostaining reagent for rabbit and diaminobenzidine (DAB) were from ZSGB-BIO (Beijing, China). Keratinocyte serum-free defined medium, epidermal growth factor (EGF), and Lipofectamine 2000 were purchased from Invitrogen. BIRC5 siGENOME small interfering (si) RNA (si-suv) was purchased from Dharmacon (Pittsburgh, PA, USA), silencer negative control #1 siRNA (si-ctrl) was from Ambion (Austin, TX, USA). Nucleus and Cytoplasm extract kit was obtained from Beyotime Biotechnology (Haimen, China). The BCA protein assay kit was purchased from Pierce Chemical (Rockford, IL, USA). Enhanced chemiluminescent (ECL) detection system was from Lulong, Inc. (Xiamen, China). Cell Counting Kit (CCK)-8 assay kit was obtained from Dojindo (Tokyo, Japan).