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Ping Chen, William Tucker, Susan Hannes, Baoying Liu, Han Si, Ankur Gupta, Richard W. J. Lee, H. Nida Sen, Robert B. Nussenblatt; Levels of Blood CD1c+ mDC1 and CD1chi mDC1 Subpopulation Reflect Disease Activity in Noninfectious Uveitis. Invest. Ophthalmol. Vis. Sci. 2015;56(1):346-352. doi: 10.1167/iovs.14-15416.
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© ARVO (1962-2015); The Authors (2016-present)
Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c+ myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.
Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.
Blood CD1c+ mDC1 and its subpopulation, CD1chi mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c+ mDC1 and CD1chi mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.
Given these observations, an alteration in blood CD1c+ mDC1 and the CD1chi mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.
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