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nurliza khaliddin, Tengku A Kamalden, Asif M Khan, Syatirah Abu Yazib, Rhuen-Chiou Chow, Samarjit Dass; First Global Assessment of Circulating microRNAs as Diagnostic and Prognostic Biomarkers for Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1042.
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To explore the role of circulating miRNAs as biomarkers and pathogenic determinants of disease progression and early detection of diabetic complications of the retina.
We have profiled circulating miRNAs in patients with DR. Total RNA was extracted from whole blood from 99 subjects (20 healthy control, Gr1; 40 diabetes without any complication, Gr2; 20 diabetes with non-proliferative DR, Gr3; and 19 diabetes with proliferative DR, Gr4). 35 candidate miRNAs were identified through microarray screening among Gr1 (pool 5 individual RNA samples) and Gr2 (pool 5 individual RNA samples) to be markers for diabetes.
Upon validation, we found that the expression levels of miR-126, miR-128 and miR-15a were significantly modulated as the DR developed and progressed. We used miR-451a (red blood cell marker) as our normalization control because with type 2 diabetes there is no change of red blood cell number. This data set is a product of a robust multivariable biostatistical analysis. Ingenuity pathway analysis suggested the miRNAs may impair angiogenic signaling, especially VEGF-dependent pathway, in patients with type 2 diabetes.
The results suggest that circulating miRNAs, in particular miR-126, miR-128 and miR-15a, may play an important role in the pathogenesis of DR. miRNAs may be potential candidates for Next Generation biomarkers.
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