April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
Role of Inflammatory CCR2+ Monocytes in Early Stage Diabetic Retinopathy
Author Affiliations & Notes
  • Alexander A Veenstra
    Pharmacology, Case Western Reserve University, Cleveland, OH
  • Timothy Kern
    Medicine (Endocrinology), Case Western Reserve University, Cleveland, OH
    Stokes Veterans Administration Medical Center, Cleveland, OH
  • Footnotes
    Commercial Relationships Alexander Veenstra, None; Timothy Kern, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1050. doi:
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      Alexander A Veenstra, Timothy Kern; Role of Inflammatory CCR2+ Monocytes in Early Stage Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1050.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Previous work demonstrated that neutrophils and perhaps monocytes play a critical role in diabetes-induced vascular lesions of the retina observed in the early stages of diabetic retinopathy. Previous attempts to create monocyte deficient animals with MCSFR-/- bone marrow were not successful in diabetic animals. Animals deficient in CCR2 do not release a sub population of “inflammatory” monocytes into the blood resulting in a monocyte deficient phenotype.

Methods: Experimental diabetes was induced in mice with streptozotocin. Diabetes-induced retinal vascular loss, leukostasis, retinal superoxide production and leukocyte activation were evaluated at durations of up to 8 months diabetes in mice lacking CCR2 and WT controls.

Results: Mice deficient in CCR2 exhibited significantly reduced diabetes-induced degeneration of retinal capillaries , leukostasis, and superoxide production. Leukocytes isolated from diabetic CCR2-/- mice exhibited reduced superoxide production and a decreased capacity to kill mouse retinal endothelial cells when co-cultured in vitro.

Conclusions: Inflammatory monocytes contribute to leukocyte mediated degeneration of retinal capillaries in diabetes, and this is mediated at least in part by CCR2

Keywords: 499 diabetic retinopathy • 557 inflammation  

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