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Ryo Ijima, Hiroki Kaneko, Fuxiang Ye, Shu Kachi, Hiroko Terasaki; Anti-histamine receptor h4 treatment reduced choroidal neovascularization independent of vascular endothelial growth factor. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1193.
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© ARVO (1962-2015); The Authors (2016-present)
To examine the relationship between vascular endothelial growth factor (Vegf) and histamine receptor h4 (Hrh4) for the treatment of choroidal neovascularization (CNV) in mice.
In wild-type and Hrh4-/- mice, laser photocoagulation was performed in the retina to induce experimental CNV (laser-CNV). Protein expressions of Vegf were examined and CNV volumes were measured in mice after inducing laser-CNV. Anti-Vegf neutralizing antibody (1μg) or isotype IgG control combined with JNJ7777120 (1μg), Hrh4 antagonist, was injected intravitreously after inducing laser-CNV, and CNV volumes were compared.
Laser-CNV volume in Hrh4-/- mice was significantly smaller than that in wild-type mice. However, mouse Vegf level in wild-type mice RPE 3 days after laser-photocoagulation was not significantly different from that in Hrh4-/- mice. Anti-VEGF neutralizing antibody mixed with JNJ7777120 significantly reduced CNV volume in wild-type mice compared to isotype IgG antibody mixed with JNJ7777120.
CNV is suppressed by Hrh4 reduction via Vegf-independent mechanism.
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