April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effect of Intravitreal Imatinib on Laser-induced Rat Model of Choroidal Neovascularization
Author Affiliations & Notes
  • Homayoun Nikkhah
    Ophthalmology, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Hamid Ahmadieh
    Ophthalmology, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Ramin Nourinia
    Ophthalmology, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Mozhgan Rezaei Kanavi
    Ophthalmology, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Bagher Hosseini
    Ophthalmology, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Mohammad Reza Oveisi
    Department of Drug and Food Control, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Naficeh Sadeghi
    Department of Drug and Food Control, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Seyed Mohsen Khandaghy Meybodi
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Mohammadreza Rahimi
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
  • Mehdi Yaseri
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1200. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Homayoun Nikkhah, Hamid Ahmadieh, Ramin Nourinia, Mozhgan Rezaei Kanavi, Bagher Hosseini, Mohammad Reza Oveisi, Naficeh Sadeghi, Seyed Mohsen Khandaghy Meybodi, Mohammadreza Rahimi, Mehdi Yaseri; Effect of Intravitreal Imatinib on Laser-induced Rat Model of Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1200.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate the safety and efficacy of intravitreal injection of a tyrosine kinase inhibitor, directed against platelet-derived growth factor and vascular endothelial growth factor receptors, in a rat model of choroidal neovascularization (CNV).

Methods: Phase I: Fifty-four pigmented rats were divided into six groups. Intravitreal injection of imatinib consisting of 330µg/5µl, 250µg/5µl, 165µg/5µl, 80µg/5µl and 40µg/5µl was performed in the right eye of each rat in groups 1 to 5 respectively. In group 6, 5µl of balanced salt solution (BSS) was injected intravitreally. Electroretinography (ERG) was performed at baseline and at weeks one and four. After euthanizing the animals, the right eyes were enucleated for histologic analysis. Based on the ERG records and histologic results, the maximum safe dose of Imatinib was determined. Phase II: Thirty-one rats were used. Experimental CNV was induced by laser photocoagulation in the right eye of each rat. In 21 rats, the highest safe dose of imatinib determined in phase I was injected intravitreally. BSS was injected in the remaining 10 rats. Fluorescein angiography was performed after 4 weeks; then the rats were euthanized and their right eyes were sent for histopathologic evaluation. Eventually, CNV area in tissue sections was measured using ImageJ software and compared between treatment and control groups.

Results: Phase I: ERG showed no statistically significant difference between Imatinib-injected eyes and the controls. However, histologic analysis disclosed focal retinal atrophic changes in the eyes receiving 165µg, 250µg, and 330µg/5µL of the drug. In the eyes that received 40µg and 80µg/5µL Imatinib, retinal histology was unremarkable and immune-reactivity for glial fibrillary acidic protein was not different from the controls. Maximum safe dose of Imatinib was determined to be 80µg/5µl. Phase II: In fluorescein angiography, there was no significant difference between the treatment and control groups in terms of leakages from CNV. Moreover, CNV area in the treatment group was not statistically different from the controls.

Conclusions: Intravitreal imatinib alone is not effective in inhibition of experimental CNV in a rat model.

Keywords: 453 choroid: neovascularization • 637 pathology: experimental • 561 injection  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×