April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
The effect of varying levels of active MMP on hydraulic conductivity across aged Bruch’s membrane.
Author Affiliations & Notes
  • Ling Zhi Heng
    Institute of Ophthalmology, London, United Kingdom
  • Ali A Hussain
    Institute of Ophthalmology, London, United Kingdom
  • John Marshall
    Institute of Ophthalmology, London, United Kingdom
  • Footnotes
    Commercial Relationships Ling Zhi Heng, None; Ali Hussain, None; John Marshall, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1320. doi:
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      Ling Zhi Heng, Ali A Hussain, John Marshall; The effect of varying levels of active MMP on hydraulic conductivity across aged Bruch’s membrane.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1320.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Both sequestration and a reduction in the free levels for activation of MMP-2 have been demonstrated in Bruch’s membrane from AMD donor eyes. In-vitro laser activation of RPE explants has shown energy-dependent increase in the release of activated-MMP2. The aim of the present study was to determine if graded increases in the level of activated MMP-2 have corresponding effects (if any) on the hydraulic conductivity of Bruch’s membrane from aged eyes.

Methods: Macular samples of human Bruch’s-choroid were obtained from 19 donors in the age range 50-80 years. These samples were mounted in open-type Ussing chambers and basal hydraulic conductivity determined. Samples were then incubated with varying levels of activated MMP-2 (1.5-30nM) followed by re-assessment of hydraulic conductivity.

Results: Compared to basal levels, hydraulic conductivity increased by 0.211+/-0.830 to 4.623+/-2.635 x10-7m-1sec -1Pa -1 in samples incubated with 1.5 and 30nM activated MMP-2 respectively. The increase in hydraulic conductivity was linearly related to the concentration of activated MMP-2 (R=0.670, p<0.05).

Conclusions: Active MMP increases hydraulic conductivity across aged Bruch’s in a concentration dependent manner. Hence, modulations of laser parameters which increase levels of active MMP 2 release can improve the function of Bruch’s membrane and must be considered when designing future clinical protocols.

Keywords: 412 age-related macular degeneration • 438 Bruch's membrane  

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