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Tsz Kin Ng, Kwong Wai Choy, Hoi Kin Wong, Chi Pui Pang, Herman S Cheung; microRNAs regulate periodontal ligament-derived stem cell retinal differentiation. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1372.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the microRNA (miRNA) signature of human adult periodontal ligament stem cells (PDLSC) retinal differentiation.
Human adult PDLSC were induced to the retinal lineage using the noggin-Dkk1-IGF-1 approach. The miRNA expression was analyzed by the miRNA microarray technique and validated by TaqMan assay. The predicted miRNA target genes were analyzed by gene ontology.
A total of 71 human miRNAs were differentially expressed before and after retinal induction, which 44 of them were upregulated and 27 were downregulated. Of the 5 selected miRNAs, 4 miRNAs (hsa-miR-132, hsa-miR-29b, hsa-miR-630 and hsa-miR-7) were validated. Gene ontology analysis of the predicted miRNA target genes confirmed the induction treatment closely related to neuronal differentiation processes.
The expression changes of miRNAs and their target genes during retinal induction process revealed the genetic and epigenetic regulatory mechanism for human adult stem cell differentiation.
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