April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
microRNAs regulate periodontal ligament-derived stem cell retinal differentiation
Tsz Kin Ng; Kwong Wai Choy; Hoi Kin Wong; Chi Pui Pang; Herman S Cheung
Author Affiliations & Notes
  • Tsz Kin Ng
    Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
    Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, FL
  • Kwong Wai Choy
    Department of Obstetrics & Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong
  • Hoi Kin Wong
    Department of Obstetrics & Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong
  • Chi Pui Pang
    Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
  • Herman S Cheung
    Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, FL
    Department of Biomedical Engineering, University of Miami, Miami, FL
  • Footnotes
    Commercial Relationships Tsz Kin Ng, None; Kwong Wai Choy, None; Hoi Kin Wong, None; Chi Pui Pang, None; Herman Cheung, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1372. doi:
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      Tsz Kin Ng, Kwong Wai Choy, Hoi Kin Wong, Chi Pui Pang, Herman S Cheung; microRNAs regulate periodontal ligament-derived stem cell retinal differentiation. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1372.

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Abstract

Purpose: To determine the microRNA (miRNA) signature of human adult periodontal ligament stem cells (PDLSC) retinal differentiation.

Methods: Human adult PDLSC were induced to the retinal lineage using the noggin-Dkk1-IGF-1 approach. The miRNA expression was analyzed by the miRNA microarray technique and validated by TaqMan assay. The predicted miRNA target genes were analyzed by gene ontology.

Results: A total of 71 human miRNAs were differentially expressed before and after retinal induction, which 44 of them were upregulated and 27 were downregulated. Of the 5 selected miRNAs, 4 miRNAs (hsa-miR-132, hsa-miR-29b, hsa-miR-630 and hsa-miR-7) were validated. Gene ontology analysis of the predicted miRNA target genes confirmed the induction treatment closely related to neuronal differentiation processes.

Conclusions: The expression changes of miRNAs and their target genes during retinal induction process revealed the genetic and epigenetic regulatory mechanism for human adult stem cell differentiation.

Keywords: 721 stem cells • 535 gene microarray  
© 2014, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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