Purchase this article with an account.
Haibin Tian, Peng Li, Li Wang, Zongyi Li, Chunpin Lian, Qingjian Ou, Lixia Lu, Weiye Li, Guo-Tong Xu; Subpopulations of monkey bone marrow mesenchymal stem cells exhibit similar therapeutic functions on retinal degeneration in RCS rats. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1387.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To provide more information about the heterogeneity of monkey bone marrow mesenchymal stem cells (BMSCs) and to confirm whether different subpopulations could be acquired by cell membrane marker CD90, and their therapeutic functions on retinal degeneration (RD) will be investigated.
Two subsets of monkey BMSCs, CD90+ and CD90-, were isolated by flow cytometry, specific surface markers were further confirmed by flow cytometry. Proliferating rate and the gene levels of growth factors and cytokines were measured by MTT method and real-time PCR. Their differentiation abilities were confirmed by culturing BMSCs in adipogenic, osteogenic and chondrogenic differentiation media. CD90+ BMSCs and CD90- BMSCs were transplanted into subretinal spaces of RCS rats, their therapeutic functions were confirmed by analyzing retinal nuclear layer thickness, apoptotic photoreceptors and electroretinogram.
Both the two CD90+ and CD90- subpopulations share similar membrane marker phenotypes, both are positive for CD73, CD44, CD29, and negative for CD45, CD34, MHCII. However, differential expression of CD90 did not influence the characteristics of the two subpopulations, regarding proliferating rate, colony forming ability, growth factor generation, adipogenic osteogenic and chondrogenic potentials. After transplanted into subretinal space of RCS rats, both CD90+ and CD90- subpopulations showed similar therapeutic functions, including increased b¬-wave amplitude, retinal nuclear layer thickness, and decreased apoptotic photoreceptors. Furthermore, when passaged in vitro, CD90- cells gradually acquired membrane molecule CD90 after two passages, which suggested that CD90- subpopulation could switch into CD90+ subpopulation when cultured in vitro.
Subpopulations could be distinguished by cell membrane markers. However, subpopulations may not always show distinct characteristics, and membrane markers are not stable when cells are cultured in vitro, which may account for their similar therapeutic functions.
This PDF is available to Subscribers Only