April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Comparing Near-Infrared and Short-Wavelength Autofluorescence in Recessive Stargardt Disease to Retinal Structure
Author Affiliations & Notes
  • Ari Dylan Schuman
    Department of Psychology, Columbia University, New York, NY
  • Tobias Duncker
    Department of Ophthalmology, Columbia University, New York, NY
  • Winston Lee
    Department of Ophthalmology, Columbia University, New York, NY
  • Rando Allikmets
    Department of Ophthalmology, Columbia University, New York, NY
    Department of Pathology & Cell Biology, Columbia University, New York, NY
  • Janet R Sparrow
    Department of Ophthalmology, Columbia University, New York, NY
    Department of Pathology & Cell Biology, Columbia University, New York, NY
  • Donald C Hood
    Department of Psychology, Columbia University, New York, NY
    Department of Ophthalmology, Columbia University, New York, NY
  • Vivienne C Greenstein
    Department of Ophthalmology, Columbia University, New York, NY
  • Footnotes
    Commercial Relationships Ari Schuman, None; Tobias Duncker, None; Winston Lee, None; Rando Allikmets, None; Janet Sparrow, None; Donald Hood, Topcon, Inc. (F); Vivienne Greenstein, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1429. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ari Dylan Schuman, Tobias Duncker, Winston Lee, Rando Allikmets, Janet R Sparrow, Donald C Hood, Vivienne C Greenstein; Comparing Near-Infrared and Short-Wavelength Autofluorescence in Recessive Stargardt Disease to Retinal Structure. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1429.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To compare hypoautofluorescent (hypoAF) areas detected with near-infrared (NIR) and short-wavelength autofluorescence (SW-AF) in patients with recessive Stargardt disease (STGD1) to retinal structure using spectral domain optical coherence tomography (OCT).

Methods: Macular volume and line scans were obtained from 7 patients (7 eyes); age 12-44 yrs with genetically confirmed STGD1, and from 15 control eyes using Spectralis HRA+OCT (Heidelberg Eng.). SW-AF (488nm) images were acquired with the Spectralis, and NIR-AF (787nm) images with the Heidelberg Retina Angiograph 2 (Heidelberg Eng). The AF images were registered to the fundus image of the OCT using i2kRetina software (DualAlign LLC). Volume scans of the patients and controls were segmented using an automated program with manual correction.[1, 2] The thicknesses of the outer segment plus (OS+) layer, from the ellipsoid zone (EZ) to the distal border of the retinal pigment epithelium, and the total retina (TR) were calculated. Thickness maps of the OCT scans and contour maps of the locations where the EZ disappeared were created. These were overlaid on their respective NIR and SW-AF images using Adobe Photoshop.

Results: All 7 patients had hypoAF areas in the central macula. In agreement with previous studies, the abnormal AF areas appeared to be larger on NIR-AF than SW-AF.[3,4] For 3 patients, the central hypoAF region on NIR-AF was surrounded by a hyperautofluorescent (hyperAF) ring which was encircled by a diffuse hypoAF area. Four patients had hypoAF flecks on NIR-AF, which were hyperAF on SW-AF. The TR and OS+ thickness maps showed progressive thinning from the periphery (+/-3 mm) towards the central fovea. Superimposition of the thickness maps on the NIR and SW-AF images showed that AF flecks and diffuse hypoAF areas outside the central atrophic lesion were associated with OS+ thinning. Superimposition of the EZ contour maps on their respective NIR and SW-AF images showed that the central hypoAF area on NIR-AF provided a better approximation of the area of EZ loss.[4]

Conclusions: These results indicate that NIR-AF and thickness maps of OCT volume scans provide additional information about the nature of the disease process in STGD1. 1. Yang et al. Biomed Opt Exp, 2011; 2. Raza et al. Arch Ophthalmol, 2011; 3. Kellner S et al. AJO, 2009; 4. Lee et al. ARVO 2013.

Keywords: 696 retinal degenerations: hereditary • 550 imaging/image analysis: clinical  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×