April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
An in vitro study of anti-inflammatory effect of Quercetin and Resveratrol polyphenols in ocular surface epithelial cells.
Author Affiliations & Notes
  • Amalia Enriquez-De-Salamanca
    Ocular Surface Group, IOBA - University of Valladolid, Valladolid, Spain
    Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain
  • Antonio Abengózar-Vela
    Ocular Surface Group, IOBA - University of Valladolid, Valladolid, Spain
    Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain
  • Carmen García-Vázquez
    Ocular Surface Group, IOBA - University of Valladolid, Valladolid, Spain
    Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain
  • Chris S Schaumburg
    Biological Sciences, Allergan Inc., Irvine, CA
  • Michael E Stern
    Biological Sciences, Allergan Inc., Irvine, CA
  • Virginia L Calder
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, United Kingdom
  • Margarita Calonge
    Ocular Surface Group, IOBA - University of Valladolid, Valladolid, Spain
    Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain
  • Maria-Jesus J. Gonzalez
    Ocular Surface Group, IOBA - University of Valladolid, Valladolid, Spain
    Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain
  • Footnotes
    Commercial Relationships Amalia Enriquez-De-Salamanca, University of Valladolid (P); Antonio Abengózar-Vela, University of Valladolid (P); Carmen García-Vázquez, None; Chris Schaumburg, Allergan Inc. (E); Michael Stern, Allergan Inc. (E), Allergan Inc. (P); Virginia Calder, Allergan Inc. (C); Margarita Calonge, Allergan Inc. (C), University of Valladolid (P); Maria-Jesus Gonzalez, University of Valladolid (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1478. doi:
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      Amalia Enriquez-De-Salamanca, Antonio Abengózar-Vela, Carmen García-Vázquez, Chris S Schaumburg, Michael E Stern, Virginia L Calder, Margarita Calonge, Maria-Jesus J. Gonzalez; An in vitro study of anti-inflammatory effect of Quercetin and Resveratrol polyphenols in ocular surface epithelial cells.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1478.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To determine the anti-inflammatory effect of quercetin (QCT) and resveratrol (RES), natural polyphenols, and their mix (QCT+RES) in two different cell lines from human conjunctival (IOBA-NHC) and corneal (HCE) epithelium.

Methods: IOBA-NHC and HCE cells were treated with QCT (0.5-25 µM), RES (0.5-50 µM), and a low dose mixture of both QCT (0.5 µM) + RES (5 µM), either in the presence or absence of 25 ng/mL tumor necrosis factor alpha (TNF-α) for 24 h. Control cells were treated with vehicle. RES and QCT cell toxicity was evaluated with alamarBlue test. Cytokine production (interleukin (IL)-6, IL-8, interferon gamma-induced protein 10 (IP-10) and vascular endothelial growth factor (VEGF)) was determined in cell supernatants by a commercial multiplex immunobead-based array. Cytokine concentration data were normalized to total amount of protein determined by the bicinchoninic acid assay in adherent cells.

Results: Stimulation of IOBA-NHC and HCE cells with TNF-α induced a significant increase of IL-6, IL-8 and IP-10 secretion in both cell lines. No signifcant TNF-α stimulation neither polyphenols effect were observed on VEGF secretion by IOBA-NHC or HCE cells. Both QCT and RES decreased TNF-α induced IL-6, and IP-10 secretion in a dose dependent manner in IOBA-NHC conjunctival and HCE corneal epithelial cells. IL-8 secretion induced by TNF-α was inhibited by QCT in both HCE (5-25 µM) and IOBA-NHC cells (20 and 25 µM); whereas it was inhibited by RES only at 50 µM dose in both cell lines. In general, the inhibitory effect of QCT and RES was greater in HCE than in IOBA-NHC, and it was also observed at lower doses of QCT than RES in both cell lines. A low dose mix of QCT+RES significantly decreased TNF-α induced IL-6 and IL-8 secretion in IOBA-NHC cells, showing a greater inhibitory effect than that exerted by both compounds separately.

Conclusions: QCT and RES have anti-inflammatory effect on both human conjunctival and corneal epithelial cell lines stimulated with TNF-α. This effect is higher when compounds are used in combination. These in-vitro data suggest that both polyphenols may have therapeutic potential in the treatment of inflammatory ocular surface diseases, such as dry eye disease or chronic ocular allergy.

Keywords: 490 cytokines/chemokines • 557 inflammation • 482 cornea: epithelium  
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