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Hiroshi Eguchi, Chika Miyazaki, Mariko Hasegawa, Masahiro Fujimoto, Yumiko Ojima, Yoshinori Mitamura; Meta-genomic analysis of secretion from acute dacryocystits. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1580.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the bacterial composition of secretion from acute dacryocystitis using 16 S rDNA clone library analysis
Six patients of acute dacryocystitis, who were seen in Tsukaguchi hospital, Hyogo, Japan, are recruited in this study. Secretions collected by aspiration from lacrimal punctum or from lacrimal sac during dacryocystorhinostomy in these patients were injected into sterilized tube. Bacterial DNAs were extracted from secretions in the tube using Extrap Soil Kit Plus ver.2 (Nippon Steel Kankyo Engineering Co., Ltd., Tokyo, Japan). The 16S rDNA gene fragments were amplified with the purified DNAs as a template and universal eubacterial 16S rDNA primer set, 27f (5'-AGAGTTTGATCMTGGCTCAG-3') and Bac1392R (5'-ACGGGCGGTGTGAC-3'). After cloning the amplified products, the cloned sequences were obtained from 24 clones using 27f as a sequencing primer. Using BLAST research, bacterial genus or species were identified.
Five in 6 cases, particular genus or species occupied the composition of the secretions as a dominant bacterium (Mycoplasma faucium, Staphylococcus aureus, Streptococcus pneumonia, Staphylococcus epidermidis, Porphyromonas sp.) In other case, 9 species were detected, and the dominant bacterium was unclear.
In most acute dacryocystitis cases, the dominant bacterium in the secretion is clear. However, some cases show polymicrobial composition of the secretions.
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