April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Safety Assessment of Chronic Suprachoroidal-Transretinal Stimulation in Normal Rabbits by the Femtosecond Laser-induced Porous Electrodes
Author Affiliations & Notes
  • Hiroyuki Tashiro
    Division of Medical Technology, Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan
  • Yasuo Terasawa
    Vision Institute, Nidek Co., Ltd., Gamagori, Japan
    Graduate School of Materials Science, Nara Institute of Science & Technology, Ikoma, Japan
  • Yukari Nakano
    Vision Institute, Nidek Co., Ltd., Gamagori, Japan
  • Koji Osawa
    Vision Institute, Nidek Co., Ltd., Gamagori, Japan
  • Motoki Ozawa
    Nidek Co., Ltd., Gamagori, Japan
  • Toshihiko Noda
    Graduate School of Materials Science, Nara Institute of Science & Technology, Ikoma, Japan
  • Takashi Tokuda
    Graduate School of Materials Science, Nara Institute of Science & Technology, Ikoma, Japan
  • Jun Ohta
    Graduate School of Materials Science, Nara Institute of Science & Technology, Ikoma, Japan
  • Takashi Fujikado
    Applied Visual Science, Osaka University Graduate School of Medicine, Suita, Japan
  • Footnotes
    Commercial Relationships Hiroyuki Tashiro, Nidek Co., Ltd. (F); Yasuo Terasawa, Nidek Co., Ltd. (E); Yukari Nakano, Nidek Co., Ltd. (E); Koji Osawa, Nidek Co., Ltd. (E); Motoki Ozawa, Nidek Co., Ltd. (E); Toshihiko Noda, Nidek Co., Ltd. (F); Takashi Tokuda, Nidek Co., Ltd. (F); Jun Ohta, Nidek Co., Ltd. (F); Takashi Fujikado, Nidek Co., Ltd. (F)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1801. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Hiroyuki Tashiro, Yasuo Terasawa, Yukari Nakano, Koji Osawa, Motoki Ozawa, Toshihiko Noda, Takashi Tokuda, Jun Ohta, Takashi Fujikado; Safety Assessment of Chronic Suprachoroidal-Transretinal Stimulation in Normal Rabbits by the Femtosecond Laser-induced Porous Electrodes. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1801. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

We have proposed the ballet-shaped platinum(Pt) electrodes with Femtosecond Laser-induced Porous surface(FLiP) [Terasawa et. al., Invest Ophthalmol Vis Sci 2012;53: E-Abstract 5538]. 24-weeks in vivo chronic stimulation studies were conducted to assess the safety of Suprachoroidal-Transretinal Stimulation(STS) with abovementioned electrodes.

 
Methods
 

An electrode array with two porous electrodes was developed for this study(Fig.1). The diameter and height of an electrode were 0.5mm and 0.3mm respectively. Femtosecond laser pulses were applied onto the surfaces of bullet-shaped electrodes to form pores. The electrode array was inserted into a scleral flap of Japanese white rabbits. Charge balanced cathodic-first biphasic pulses with 1.5mA amplitude and 0.5ms width at repetition frequency of 50Hz were applied to one of two electrodes on the array for 8 hours per day for 24 weeks. Examinations including fundus photography, optical coherence tomography(OCT), electrically evoked potentials(EEPs) and histological evaluation were performed to detect tissue damage. Explanted stimulation electrodes were observed with scanning electron microscope(SEM) to compare with images obtained before implantation. All procedures conformed to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.

 
Results
 

No obvious injuries were observed in the all examination(Fig. 2). The EEP responses were observed after 24-weeks chronic stimulation. No sign of degradation was observed in the SEM of explanted electrodes.

 
Conclusions
 

We succeeded in confirming the safety of chronic STS with the FLiP electrodes. Additionally the electrodes showed enough reliability and biocompatibility for chronic stimulation in vivo.

  
Keywords: 688 retina  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×