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Fang Liu, Yan Su, Fang Wang; Experimental study on intervention of choroidal neovascularization by anti-osteopontin antibody. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1805.
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To investigate the mechanism of inhibiting the CNV formation through blocking OPN by using anti-OPN antibody.
Laser-induced CNV model was created in 40 eyes of 40 male SPF C57BL/6J mice by Argon laser with the wavelength 514nm. 36 successful CNV model mice were randomly divided into anti-OPN antibody group (group A), M-IgG group (group B) and PBS group (group C), including 12 mice in each group. The mice in each group were received intraperitoneal injection of 400 ug of anti-OPN antibody, M-IgG and PBS respectively on the second days after modeling. The CNV was evaluated by using fundus fluorescein angiography (FFA) on the seventh days after modeling. The mice were immediately sacrificed and the eyeballs were enucleated on the fourteenth day after modeling. 4 eyeballs in each group were used to observe the areas of CNV by RPE-choroid-sclera flat mounts. The rest eyeballs were used to analyze OPN,VEGF expression by using fluorescence quantitative polymerase chain reaction (QF-PCR).
FFA Showed that there was fluorescein leakage in photocoagulation areas after 7 days photocoagulation , thus it can be confirmed CNV formation .The CNV areas were shown (16.98±0.70)×103,(27.13±0.81)×103,(35.39±2.14)×103μm2 respectively in each group, with a significant difference (F=533.76, P=0.000). The expressions of VEGF in choroidal were significant lower in group A than that in group B (t=-6.74,P<0.01), while the expressions of OPN were not significant difference in each group (t=-5.26, P>0.05).
Anti-OPN antibody can inhabit the formation of CNV in laser-induced mouse model by down-regulating VEGF.
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