April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Chlamydia infection status in trachoma trichiasis patients in two different trachoma endemic countries
Author Affiliations & Notes
  • Barisani-Asenbauer Talin
    LB Centre of Expertise OCUVAC, Medical University of Vienna, Vienna, Austria
  • Hadeel Aichalabi
    LB Centre of Expertise OCUVAC, Medical University of Vienna, Vienna, Austria
  • Lamiss Mejdoubi
    LB Centre of Expertise OCUVAC, Medical University of Vienna, Vienna, Austria
  • Aleksandra Inic-Kanada
    LB Centre of Expertise OCUVAC, Medical University of Vienna, Vienna, Austria
  • Elisabeth Stein
    LB Centre of Expertise OCUVAC, Medical University of Vienna, Vienna, Austria
  • Nadine Schuerer
    LB Centre of Expertise OCUVAC, Medical University of Vienna, Vienna, Austria
  • Yeshigeta Belaw
    Jimma University, Jimma, Ethiopia
  • Balgesa Elkheir
    Federal Ministry of Health, Khartoum, Sudan
  • Tanja Cirkovic Velickovic
    University of Belgrade, Belgrade, Serbia
  • Gerold Stanek
    Center for Pathophysiology, Immunology and Infectiology, Medical University of Vienna, Vienna, Austria
  • Footnotes
    Commercial Relationships Barisani-Asenbauer Talin, None; Hadeel Aichalabi, None; Lamiss Mejdoubi, None; Aleksandra Inic-Kanada, None; Elisabeth Stein, None; Nadine Schuerer, None; Yeshigeta Belaw, None; Balgesa Elkheir, None; Tanja Cirkovic Velickovic, None; Gerold Stanek, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1861. doi:https://doi.org/
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      Barisani-Asenbauer Talin, Hadeel Aichalabi, Lamiss Mejdoubi, Aleksandra Inic-Kanada, Elisabeth Stein, Nadine Schuerer, Yeshigeta Belaw, Balgesa Elkheir, Tanja Cirkovic Velickovic, Gerold Stanek; Chlamydia infection status in trachoma trichiasis patients in two different trachoma endemic countries. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1861. doi: https://doi.org/.

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Abstract

Purpose: The aim of the study was to characterize the chlamydial infection status of subjects with trachomatous trichiasis (TT) and trachoma negative controls in two different trachoma endemic regions.

Methods: 100 patients scheduled for trichiasis surgery (TT) and 100 matched controls (CO) without signs of trachoma were randomly selected in an ophthalmology department in Jimma, Ethiopia and in a field surgery clinic in Sudan. qPCR was used to detect genomic DNA for Chlamydiaceae in conjunctival swabs and urine samples. Antibody responses to Chlamydiae pneumoniae (ChPn), Chlamidiae trachomatis (ChT), chlamydial LPS and ChT-Hsp60 were determined in sera. 1D SDS-PAGE was performed against ChT proteins in 20 TT and 20 CO.

Results: 95,4% of TT vs. 91,4% of CO were found positive for anti-ChPn IgG . 5,8% of TT vs. 1,2% of CO were found positive for anti-ChPn IgM, and 36,8% vs 29,6% for anti-ChPn IgA. 37,93% of TT vs 26% of CO were positive for anti-ChT IgG and 12,6% vs 9,88% for anti-ChT IgA. IgG Abs against ChT Hsp60 were detected in 78,1% of TT vs 75,3% of CO. 1D SDS-PAGE revealed differences in antigen mapping profiles between TT and negative subjects.

Conclusions: No assocation between ChT-Hsp60 antibodies and TT as well as high positivity rates for ChPn antibodies in both groups were found.

Keywords: 736 trachoma • 433 bacterial disease  
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