April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Retinal Ganglion Cells Expressing Melanopsin Are Injury-Resistant After Retinal Ischemia.
Author Affiliations & Notes
  • Ruth Estela Rosenstein
    Dept Human Biochem-Sch Med, University of Buenos Aires, Buenos Aires, Argentina
  • Maria F Gonzalez Fleitas
    Dept Human Biochem-Sch Med, University of Buenos Aires, Buenos Aires, Argentina
  • Marcos L Aranda
    Dept Human Biochem-Sch Med, University of Buenos Aires, Buenos Aires, Argentina
  • Nuria de Zavalia
    Dept Human Biochem-Sch Med, University of Buenos Aires, Buenos Aires, Argentina
  • Pablo Sande
    Dept Human Biochem-Sch Med, University of Buenos Aires, Buenos Aires, Argentina
  • Damian Dorfman
    Dept Human Biochem-Sch Med, University of Buenos Aires, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships Ruth Rosenstein, None; Maria Gonzalez Fleitas, None; Marcos Aranda, None; Nuria de Zavalia, None; Pablo Sande, None; Damian Dorfman, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1895. doi:https://doi.org/
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      Ruth Estela Rosenstein, Maria F Gonzalez Fleitas, Marcos L Aranda, Nuria de Zavalia, Pablo Sande, Damian Dorfman; Retinal Ganglion Cells Expressing Melanopsin Are Injury-Resistant After Retinal Ischemia.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1895. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We investigated the effect of acute retinal ischemia on the non-image forming visual system, particularly on melanopsin expressing retinal ganglion cells (RGC).

Methods: Ischemia was induced in male Wistar rats by increasing intraocular pressure (120 mm Hg for 40 min). Retinal function (ERG), the number of Brn3a(+) and melanopsin(+) RGC (immunohistochemistry), and melanopsin levels (Western Blot), as well as the pupil light reflex (PRL) (after 30-s light flash) were examined. Anterograde transport was examined after an intravitreal injection of cholera toxin β-subunit, and circadian rhythms of general locomotor activity were registered in cages equipped with infrared detectors of motion.

Results: After 4 weeks of ischemia, clear alterations in the visual function and retinal histology were observed. Concomitantly with a significant decrease in the number of Brn3a(+) RGC, no differences in the number of melanopsin(+) cells, and melanopsin levels were observed between non-ischemic and ischemic retinas. Ischemia decreased retinal projections to the superior colliculus, whereas the anterograde transport to the suprachiasmatic nucleus and the olivary pretectal nucleus remained unaffected. No differences in PRL were observed between control and ischemic eyes, and the locomotor activity pattern was conserved in animals submitted to bilateral ischemia.

Conclusions: These results indicate melanopsin(+) RGC, and the non-image forming visual system are resistant to ischemic injury.

Keywords: 572 ischemia • 458 circadian rhythms • 531 ganglion cells  
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