April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Placental Growth Factor Levels Correlated with Retinal Ischemia Progression
Author Affiliations & Notes
  • Kyle V Marra
    Ophthalmology, Beth Israel Deaconess Medical Center, Brighton, MA
  • Kyle Kovacs
    Albert Einstein College of Medicine, Bronx, NJ
  • Sushant Wagley
    College of Human Medicine, Michigan State University, East Lansing, NJ
  • Sudheer Akella
    Ophthalmology, Beth Israel Deaconess Medical Center, Brighton, MA
  • Gianna C Teague
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye & Ear, Boston, MA
  • Walter Johnson
    Physics, Suffolk University, Boston, MA
  • Kameran Lashkari
    Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye & Ear, Boston, MA
  • Jorge G Arroyo
    Ophthalmology, Beth Israel Deaconess Medical Center, Brighton, MA
  • Footnotes
    Commercial Relationships Kyle Marra, None; Kyle Kovacs, None; Sushant Wagley, None; Sudheer Akella, None; Gianna Teague, None; Walter Johnson, None; Kameran Lashkari, None; Jorge Arroyo, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1909. doi:https://doi.org/
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      Kyle V Marra, Kyle Kovacs, Sushant Wagley, Sudheer Akella, Gianna C Teague, Walter Johnson, Kameran Lashkari, Jorge G Arroyo; Placental Growth Factor Levels Correlated with Retinal Ischemia Progression. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1909. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To characterize the vitreous cytokine, chemokine, and growth factor profiles in patients with increasing retinal ischemia.

 
Methods
 

This IRB-approved study retrospectively analyzed 81 undiluted vitreous samples obtained from patients undergoing pars plana vitrectomy by a single surgeon at Beth Israel Deaconess Medical Center from November 2010 to September 2012. The specimens underwent a Bio-Plex Pro Human Cytokine Assay to determine the levels of 34 proteins including chemokines, cytokines, non-inflammatory proteins, and growth factors. Specimens were divided into the following four groups based on whether the patient underwent: 1.) vitrectomy for epiretinal membrane peeling and/or macular hole with no history of diabetes (control group), 2) vitrectomy for epiretinal membrane peeling and/or macular hole with a history of diabetes (DM group), 3) vitrectomy for proliferative diabetic retinopathy (PDR group), and 4) vitrectomy for neovascular glaucoma (NVG group). Parametric and non-parametric analyses were performed using SPSS software comparing demographics, as well as protein levels between each group.

 
Results
 

There was no significant difference in age and gender between groups. Numerous proteins were noted to be significantly elevated comparing the control and DM group (G-CSF, sCD40L, Endoglin, IL-6, PlGF, VEGF-D), the DM and PDR group (leptin, IL-8, PlGF, VEGF-A), as well as the DM to NVG group (G-CSF, leptin, TIE-2, sCD40L, EGF, HB-EGF, IL-6, IL-8, PlGF, TNF-alpha). Of note, placental growth factor (PlGF) exhibited a significant increase in all the aforementioned comparisons. Most proteins elevated in the PDR and NVG groups were significantly elevated compared to the control group as well.

 
Conclusions
 

Both angioproliferative growth factors as well as inflammatory proteins are elevated in eyes with severe retinal ischemia. We found that vitreous levels of PlGF increase significantly in patients with worsening retinal ischemia.

 
Keywords: 490 cytokines/chemokines • 572 ischemia • 688 retina  
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