April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
In Vivo and In Vitro Imaging of Pigmented Structures in the Rat Eye with Polarization Sensitive Optical Coherence Tomography
Author Affiliations & Notes
  • Bernhard Baumann
    Ctr for Med Physics & Biomed Engineering, Medical University of Vienna, Vienna, Austria
  • Sabine Rauscher
    Core Facility Imaging, Medical University of Vienna, Vienna, Austria
  • Martin Glösmann
    Core Facility for Research and Technology, University of Veterinary Medicine Vienna, Vienna, Austria
  • Johannes Schirmer
    Ctr for Med Physics & Biomed Engineering, Medical University of Vienna, Vienna, Austria
  • Stanislava Fialova
    Ctr for Med Physics & Biomed Engineering, Medical University of Vienna, Vienna, Austria
  • Erich Götzinger
    Ctr for Med Physics & Biomed Engineering, Medical University of Vienna, Vienna, Austria
  • Michael Pircher
    Ctr for Med Physics & Biomed Engineering, Medical University of Vienna, Vienna, Austria
  • Marion Gröger
    Core Facility Imaging, Medical University of Vienna, Vienna, Austria
  • Christoph K Hitzenberger
    Ctr for Med Physics & Biomed Engineering, Medical University of Vienna, Vienna, Austria
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2101. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Bernhard Baumann, Sabine Rauscher, Martin Glösmann, Johannes Schirmer, Stanislava Fialova, Erich Götzinger, Michael Pircher, Marion Gröger, Christoph K Hitzenberger; In Vivo and In Vitro Imaging of Pigmented Structures in the Rat Eye with Polarization Sensitive Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2101.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

To demonstrate polarization sensitive (PS) optical coherence tomography (OCT) for investigating pigmentation in the eyes of different rat strains in vivo. To correlate the findings in PS-OCT images to histological analysis of the same tissues.

 
Methods
 

A prototype high-speed PS-OCT system was modified for imaging the rat eye. Healthy male rats of both pigmented (Brown Norway, Long Evans) and albino (Sprague Dawley) strains were anaesthetized and densely sampled PS-OCT raster scans covering an area of ~1.5 mm x 1.5 mm centered at the papilla were acquired. In addition to conventional OCT images based on reflectivity, cross-sectional PS-OCT images displaying the birefringent and depolarizing tissue properties were computed from the 3D data of the ocular structures. The eyes were enucleated post mortem for histological analysis. After fixation, cryosectioning and staining, confocal microscopy imaging of ocular structures in the posterior globe was performed. In vitro PS-OCT imaging was performed in unstained histological sections in order to compare tissue appearance to histology.

 
Results
 

In reflectivity images, the retinal layers and the choroid can be observed. In the albino rat eye, light penetration through the non-pigmented choroid is increased and the backscatter signal from the sclera is stronger. Depolarization was investigated in degree of polarization uniformity (DOPU) images. High DOPU values indicating uniform polarization characteristics were observed in most tissues. Depolarization, i.e. low DOPU values, was observed in structures containing melanin granules, namely the RPE/choroid complex in Brown Norway and Long Evans rats. In addition, the remnant of the hyaloid artery exhibited depolarization in the Brown Norway rat retina. Overlap of depolarization and melanin pigmentation in the RPE/choroid complex and the hyaloid artery remnant was confirmed using histological analysis.

 
Conclusions
 

PS-OCT enables quantitative imaging of polarizing tissue properties in addition to conventional OCT imaging based on reflectivity. Ocular structures containing melanin granules appeared depolarizing in PS-OCT images. Since pigmented structures such as the RPE and the choroid play key roles in age-related macular degeneration (AMD), PS-OCT may provide a promising technology for in vivo imaging of (micro)structural changes in disease models of AMD.

  
Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 596 microscopy: confocal/tunneling  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×