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Shoko Ishida, Fumiki Okamoto, Sujin Hoshi, Shinichi Fukuda, Yoshimi Sugiura, Mikki Arai, Tatsuo Hirose, Tetsuro Oshika; FocalSeal® for Closure of Sutureless Sclerotomies of Vitrectomy: An In Vivo and Histological Study. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2337.
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© ARVO (1962-2015); The Authors (2016-present)
FocalSeal® is an absorbable polyethylene glycol-based synthetic hydrogel sealant. This liquid is polymerized under visible xenon illumination, and forms clear, flexible, and firmly adherent hydrogel. In this study, we evaluated the ability of FocalSeal® to close sclerotomies of microincision vitrectomy.
Dutch pigmented normal rabbits were used in this in vivo study. We performed 23-gauge 3-port vitrectomy on a unilateral eye of each study animal. The sclerotomy was made by inserting a 23-gauge microvitreoretinal knife at an angle of 45° transconjunctivally. After core vitrectomy, vitreous gel around the cannula was removed with the vitrectomy cutter and then fluid-air exchange was performed. After cannulas except for irrigation were removed, 0.625% Povidone-iodine was dropped over the conjunctiva on the sclerotomy site to confirm air leakage from the sclerotomy. Then, FocalSeal® was injected subconjunctivally to cover the sclerotomies, and was polymerized with 60-second application of xenon light. Povidone-iodine was again dropped over the conjunctiva on the sclerotomy site to check whether air leakage from the sclerotomies exists. The eyes were enucleated 7 days after operation for histological examinations.
After subconjunctival injection and application of xenon light, FocalSeal® polymerized rapidly. No air leakage was observed when povidone-iodine was dropped over the sclerotomy site. On histological examination, linear scar formations were observed at the sclerotomy sites and sclerotomy tunnels were tightly closed.
This experiment suggests that FocalSeal® allows secure closure of sutureless vitrectomy incisions.
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