April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Immunosuppressive function of exosomes isolated from circulating blood of the mice with experimental autoimmune uveitis (EAU)
Author Affiliations & Notes
  • Guomin Jiang
    Department of Ophthalmology, University of Louisville, Louisville, KY
  • Amir Reza Hajrasouliha
    Department of Ophthalmology, University of Louisville, Louisville, KY
  • Yunsong Wang
    Department of Ophthalmology, University of Louisville, Louisville, KY
  • Henry J Kaplan
    Department of Ophthalmology, University of Louisville, Louisville, KY
  • Hui Shao
    Department of Ophthalmology, University of Louisville, Louisville, KY
  • Footnotes
    Commercial Relationships Guomin Jiang, None; Amir Hajrasouliha, None; Yunsong Wang, None; Henry Kaplan, None; Hui Shao, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2494. doi:
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    • Get Citation

      Guomin Jiang, Amir Reza Hajrasouliha, Yunsong Wang, Henry J Kaplan, Hui Shao; Immunosuppressive function of exosomes isolated from circulating blood of the mice with experimental autoimmune uveitis (EAU). Invest. Ophthalmol. Vis. Sci. 2014;55(13):2494.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To determine whether exosomes isolated from the sera of EAU mice contain uveitogenic interphotoreceptor petinoid binding protein (IRBP), and whether these IRBP-containing exosomes are immunogenic or tolerogenic.

Methods: EAU was induced in C57BL/6 (B6) mice by immunization with IRBP1-20 peptides emulsified with complete Freund’s adjuvant. Exosomes were extracted from the sera of naïve and diseased mice by ultra-speed centrifuge. The presence of IRBP in the exosomes was examined by western blot using anti-IRBP Ab. IRBP-specific T cell responses after incubation with IRBP containing exosomes were tested by T cell proliferation assay. The severity of EAU treated with or without IRBP-containing exosomes was evaluated by histology.

Results: Exosomes from the sera of EAU mice expressed significantly high level of IRBP compared to those from naïve mice. These IRBP containing exosomes inhibited the proliferation of uveitogenic T cells in vitro and suppressed the uveitis induced by IRBP-specific T cells in vivo.

Conclusions: IRBP-containing exosomes was detected in the peripheral blood, which might deliver immunosuppressive signals to autoimmune T cells and might be used as a novel therapy for autoimmune uveitis.

Keywords: 432 autoimmune disease • 745 uvea • 555 immunomodulation/immunoregulation  
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