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Guomin Jiang, Amir Reza Hajrasouliha, Yunsong Wang, Henry J Kaplan, Hui Shao; Immunosuppressive function of exosomes isolated from circulating blood of the mice with experimental autoimmune uveitis (EAU). Invest. Ophthalmol. Vis. Sci. 2014;55(13):2494.
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© ARVO (1962-2015); The Authors (2016-present)
To determine whether exosomes isolated from the sera of EAU mice contain uveitogenic interphotoreceptor petinoid binding protein (IRBP), and whether these IRBP-containing exosomes are immunogenic or tolerogenic.
EAU was induced in C57BL/6 (B6) mice by immunization with IRBP1-20 peptides emulsified with complete Freund’s adjuvant. Exosomes were extracted from the sera of naïve and diseased mice by ultra-speed centrifuge. The presence of IRBP in the exosomes was examined by western blot using anti-IRBP Ab. IRBP-specific T cell responses after incubation with IRBP containing exosomes were tested by T cell proliferation assay. The severity of EAU treated with or without IRBP-containing exosomes was evaluated by histology.
Exosomes from the sera of EAU mice expressed significantly high level of IRBP compared to those from naïve mice. These IRBP containing exosomes inhibited the proliferation of uveitogenic T cells in vitro and suppressed the uveitis induced by IRBP-specific T cells in vivo.
IRBP-containing exosomes was detected in the peripheral blood, which might deliver immunosuppressive signals to autoimmune T cells and might be used as a novel therapy for autoimmune uveitis.
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