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Kyungmin Lee, Abhishek R Payal, Jamie Metzinger, Reena Rasheed, Ninani Kombo, Pranav Patel, Homaira Ayesha Hossain, C Stephen Foster; Assessment of Inflammatory Cytokines in Aqueous Humour of Patients with Sarcoidosis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2514.
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© ARVO (1962-2015); The Authors (2016-present)
To compare the concentrations of 20 cytokines from T helper (Th) pathways (Th1, Th2, and Th17) in aqueous humour of patients with sarcoid uveitis (active or in remission) to normal eyes.
Single center, prospective, observational case control study. Aqueous samples were collected from 7 eyes with sarcoid uveitis (SU group), and 8 normal eyes (control group). Quantitative multiplex sandwich ELISA-based microarray assay was used to quantify 20 cytokines: interleukin (IL)-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17, IL-17f, IL-21, IL-22, IL-23, IL-28a, macrophage inflammatory protein-3α (MIP-3α), transforming growth factor-β1 (TGF-β), interferon-γ (IFN-γ), granulocyte macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α) and tumor necrosis factor-β (TNF-β). Cytokine levels from patients were compared with normal controls.
The aqueous levels of IL-2 (10.6 pg/ml: 1.5-40.5, p: 0.024) and TNF-α (98.5 pg/ml: 4.2-542.6, p: 0.0093) were significantly higher in the sarcoidosis group compared with the control group. The aqueous levels of IL-2, IL-6, IL-21, IL-23, and GM-CSF were higher in the active uveitis patients compared to the inactive group. TNF-α was higher in the remission patients.
Multiplex immunoassay of 20 cytokines in patients with sarcoid associated uveitis revealed that IL-2 and TNF- α were concentrated in the aqueous fluid. We confirm that IL-21 and IL-23 levels were elevated and correlated with disease activity, suggesting Th17 cells play an important role in autoimmune disease and uveitis.
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