April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
In Vivo Microscopic Assessment Of Perfused Foveal Capillary Lumen Diameters In Diabetic Retinopathy Versus Healthy Controls
Author Affiliations & Notes
  • Richard Donald Bavier
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
  • Moataz M Razeen
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
  • Alexander Gan
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
  • Alexander Pinhas
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
    Icahn School of Medicine at Mount Sinai, New York, NY
  • Nishit Shah
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
  • Eric Cheang
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
    Bronx High School of Science, Bronx, NY
  • Chun Lin Liu
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
    Stuyvesant High school, New York, NY
  • Toco Yuen Ping Chui
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
  • Alfredo Dubra
    Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, WI
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI
  • Richard B Rosen
    Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY
    Department of Ophthalmology, New York Medical College, Valhalla, NY
  • Footnotes
    Commercial Relationships Richard Bavier, None; Moataz Razeen, None; Alexander Gan, None; Alexander Pinhas, None; Nishit Shah, None; Eric Cheang, None; Chun Lin Liu, None; Toco Chui, None; Alfredo Dubra, Canon USA Inc (C), US Patent No: 8,226,236 (P); Richard Rosen, Clarity (C), OD-OS (C), Optovue (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2602. doi:
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      Richard Donald Bavier, Moataz M Razeen, Alexander Gan, Alexander Pinhas, Nishit Shah, Eric Cheang, Chun Lin Liu, Toco Yuen Ping Chui, Alfredo Dubra, Richard B Rosen; In Vivo Microscopic Assessment Of Perfused Foveal Capillary Lumen Diameters In Diabetic Retinopathy Versus Healthy Controls. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2602.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To measure the lumen diameter of perfused capillaries surrounding the foveal avascular zone (FAZ) and assess the variability in diabetic retinopathy (DR) versus healthy control eyes.

 
Methods
 

Offset Pinhole (OP) Adaptive Optics scanning light ophthalmoscopy(AOSLO) imaging was performed on 5 subjects with DR (mean age 47, 35-61 years old) and 4 healthy controls (mean age 29, 25-37 years old). OP AOSLO used a 790 nm light source and a 1.5° field-of-view. Whenever used, AOSLO FA was performed using simultaneous reflectance (790 nm) and fluorescence (488 nm) image sequences with a 1.75° field-of-view after consumption of oral fluorescein. After sinusoidal distortion and eye motion correction, respective registered averages were stitched together to create structural (OP AOSLO) and perfusion (AOSLO FA) maps. Structural and perfusion maps were compared to identify the perfused capillaries surrounding the FAZ (Fig. 1). To ensure equal distribution of measurements, structural maps were divided into equiangular octants and segments 15° apart centered at the fovea. Lumen diameter measurements on perfused capillaries in OP AOSLO structural maps were then performed manually on 3 segments per octant.

 
Results
 

Results are presented as mean diameters± SD, and statistical significance was assessed using a two-tail t-test with a p-value <0.05. DR eyes had statistically significant higher perifoveal perfused capillary lumen diameters than those of control eyes (5.5 ± 2.2 μm vs. 4.2 ± 0.89 μm; t(204) = 5.2, p < 0.0001). Also, DR eyes exhibited greater lumen diameter variability with a larger coefficient of variation compared to that of control eyes (0.34 vs. 0.24).

 
Conclusions
 

The ability to quantify changes to the FAZ capillary lumen diameter in subjects with DR may have clinical value for early detection of microvascular changes, enabling interventions prior to capillary decompensation.

 
 
Fig. 1 (a) Fluorescein Angiography perfusion map of FAZ capillaries* (b) Offset pinhole structural map of FAZ capillaries* *non-perfused capillaries are denoted with white arrows
 
Fig. 1 (a) Fluorescein Angiography perfusion map of FAZ capillaries* (b) Offset pinhole structural map of FAZ capillaries* *non-perfused capillaries are denoted with white arrows
 
Keywords: 688 retina • 499 diabetic retinopathy • 550 imaging/image analysis: clinical  
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