April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Factors associated with thickness of eye bank-prepared DSAEK graft tissue
Author Affiliations & Notes
  • Grace M Wang
    University of Michigan Kellogg Eye Center, Ann Arbor, MI
  • Maria A Woodward
    University of Michigan Kellogg Eye Center, Ann Arbor, MI
  • Michael O'Keefe
    Midwest Eye-Banks, Ann Arbor, MI
  • Indu Vadakkepattath
    Midwest Eye-Banks, Ann Arbor, MI
  • Michael Titus
    Saving Sight, Columbus, MO
  • Taylor Blachley
    University of Michigan Kellogg Eye Center, Ann Arbor, MI
  • David C Musch
    University of Michigan Kellogg Eye Center, Ann Arbor, MI
  • Roni M Shtein
    University of Michigan Kellogg Eye Center, Ann Arbor, MI
  • Footnotes
    Commercial Relationships Grace Wang, None; Maria Woodward, None; Michael O'Keefe, Midwire (E); Indu Vadakkepattath, Midwire (E); Michael Titus, Saving Sight (E); Taylor Blachley, None; David Musch, None; Roni Shtein, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2697. doi:https://doi.org/
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    • Get Citation

      Grace M Wang, Maria A Woodward, Michael O'Keefe, Indu Vadakkepattath, Michael Titus, Taylor Blachley, David C Musch, Roni M Shtein; Factors associated with thickness of eye bank-prepared DSAEK graft tissue. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2697. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To identify factors associated with thickness of eye bank-prepared DSAEK grafts.

Methods: Data from all corneal tissues processed for DSAEK at a single eye bank from 2008-2012 were included (n= 7,668). Multivariate linear regression modeling was used to identify factors contributing to thickness of DSAEK grafts.

Results: Eye bank-prepared DSAEK grafts have become thinner over time, on average 48 microns thinner in 2012 than in 2008. Decreased pre-processing corneal thickness and higher pre-processing endothelial cell density were associated with thinner lamellar grafts (p<0.0001), but post-processing cell density and graft diameter were not associated with graft thickness (p=0.70 and p=0.88). Older donor age was associated with thinner grafts (p<0.0001). There was no significant interaction between donor age and endothelial cell density in relation to final graft thickness (p=0.161). Death-to-procurement time and death-to-processing time were not associated with graft thickness (p=0.94 and p=0.93). DSAEK grafts processed during the “work-day” (8a-5p) were on average 3.8 microns thinner than those processed “after-hours” (5p-8a; p<0.0001). In comparing technician experience, no significant difference was found between number of tissues processed and post-processing thickness.

Conclusions: Eye bank-prepared DSAEK graft thickness has decreased over time, and thinner grafts were associated with thinner corneal tissue, increased endothelial cell density, increased donor age, and daytime tissue preparation. There was no association between graft thickness and death-to-procurement time, death-to-processing time, or technician experience.

Keywords: 479 cornea: clinical science • 741 transplantation  
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