April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Measurement of Peripheral Non-perfusion in Ultra wide-field fluorescein angiography
Author Affiliations & Notes
  • Jedediah McClintic
    Ophthalmology, Cleveland Clinic Cole Eye Inst, Cleveland, OH
  • Peter K Kaiser
    Ophthalmology, Cleveland Clinic Cole Eye Inst, Cleveland, OH
  • Sunil K Srivastava
    Ophthalmology, Cleveland Clinic Cole Eye Inst, Cleveland, OH
  • Rishi P Singh
    Ophthalmology, Cleveland Clinic Cole Eye Inst, Cleveland, OH
  • Justis P Ehlers
    Ophthalmology, Cleveland Clinic Cole Eye Inst, Cleveland, OH
  • Footnotes
    Commercial Relationships Jedediah McClintic, None; Peter Kaiser, None; Sunil Srivastava, None; Rishi Singh, None; Justis Ehlers, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 270. doi:
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    • Get Citation

      Jedediah McClintic, Peter K Kaiser, Sunil K Srivastava, Rishi P Singh, Justis P Ehlers; Measurement of Peripheral Non-perfusion in Ultra wide-field fluorescein angiography. Invest. Ophthalmol. Vis. Sci. 2014;55(13):270.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Ultra-widefield fluorescein angiography has allowed the evaluation of peripheral non perfusion in retinal vascular disease such as diabetic retinopathy and retinal vein occlusions. The purpose of this study was to compare measurements of peripheral retinal ischemia using ischemic index on standard images and stereographic corrected projection images created with Optos Optomap ® software and to compare these measurements to the Optomap area measurement tool in patients with branch retinal vein occlusions

 
Methods
 

Retrospective chart review of patients with branch retinal vein occlusions who received ultra-widefield angiography in the preceding 1 year. Quantitative analysis of ischemic burden was performed using Image J software. Areas of ischemia were identified and measured against the total area to compute an ischemic index as described by Schwartz et al. Images were then processed using Optos Optomap software that adjusts image presentation to a best-fit 24 mm globe and projects a flat map which preserves peripheral aspect ratios. Ischemic index was calculated again using these images. Direct measurement of ischemic retinal area was performed using the Optos area measurement tool.

 
Results
 

Ultra-widefield fluorescein angiography from 8 eyes of eight patients diagnosed with BRVO who had not been treated with laser photocoagulation were analyzed in both standard and Optos Optomap ® projections. Total area of peripheral nonperfusion measured using Image J software was, on average, 72% larger on standard images than Optomap stereoscopic projections (P = 0.015). However, the ischemic index of visualized retina trended larger (7.6%) using Optomap projections than standard images (P = 0.08).

 
Conclusions
 

Ultra-widefield fluorescein angiography has allowed for the assessment of peripheral non perfusion in various disease states. Image distortion can be variable and introduces significant error when interpreting the dimension of ischemia. Current ultra-widefield imaging typically over-represents peripheral retina. Stereoscopic map projections of the retina allow for more accurate analyses of non perfusion and will promote specificity in characterizing the impact of ischemia on clinical endpoints such as macular edema and neovascularization.

 
 
Standard wide-field image
 
Standard wide-field image
 
 
Optomap projection image
 
Optomap projection image
 
Keywords: 549 image processing • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 688 retina  
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