April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Polymorphism -1486T/C TLR 9 gene as a risk factor in infectious blepharitis
Author Affiliations & Notes
  • Daniela Garcia Romero
    Cornea, Hospital de la luz, Mexico, Mexico
  • Alejandro Babayan
    Cornea, Hospital de la luz, Mexico, Mexico
  • Oscar Fernandez
    Cornea, Hospital de la luz, Mexico, Mexico
  • Karen Tellez-Alcaide
    Centro de Investigación Biomédica, Hospital de la luz, Mexico, Mexico
  • Atzin Robles-Contreras
    Centro de Investigación Biomédica, Hospital de la luz, Mexico, Mexico
  • Hector Javier Perez-Cano
    Centro de Investigación Biomédica, Hospital de la luz, Mexico, Mexico
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2749. doi:
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      Daniela Garcia Romero, Alejandro Babayan, Oscar Fernandez, Karen Tellez-Alcaide, Atzin Robles-Contreras, Hector Javier Perez-Cano, Hospital de la luz; Polymorphism -1486T/C TLR 9 gene as a risk factor in infectious blepharitis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2749.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To demonstrate the association between polymorphism of -1486T/C TLR9 gene promoter region with infectious blepharitis.

Methods: Thirty samples of palpebral scraping from patients with infectious blepharitis, and thirty samples from not infected subjects, as controls, were studied. The promoter region of toll like receptor 9 (TLR 9) gene was amplified by PCR, and then, the PCR products were purified and analyzed the presence of SNP -1486T/C by direct sequencing. Fisher Chi-square was used to found the association between blepharitis and the polymorphism in the promoter region.

Results: The allelic frequency of -1486T was 62.5% of the promoter region of TLR 9 gene in patients, and in controls was 25%. Our results showed significantly differences between patients and control subjects (p<0.0001) and the relative risk was 2.48 and the Odds Ratio 4.89.

Conclusions: In this study of Mexican population on infectious blepharitis and polymorphism of the promoter region of TLR 9 gene, we found high frequency for -1486T allele in patients with infectious blepharitis,which indicates that there is relationship between -1486T polymorphism in TLR 9 gene and is associated with the susceptibility for infectious blepharitis in the studied population.

Keywords: 480 cornea: basic science  
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