Abstract
Purpose:
To demonstrate the association between polymorphism of -1486T/C TLR9 gene promoter region with infectious blepharitis.
Methods:
Thirty samples of palpebral scraping from patients with infectious blepharitis, and thirty samples from not infected subjects, as controls, were studied. The promoter region of toll like receptor 9 (TLR 9) gene was amplified by PCR, and then, the PCR products were purified and analyzed the presence of SNP -1486T/C by direct sequencing. Fisher Chi-square was used to found the association between blepharitis and the polymorphism in the promoter region.
Results:
The allelic frequency of -1486T was 62.5% of the promoter region of TLR 9 gene in patients, and in controls was 25%. Our results showed significantly differences between patients and control subjects (p<0.0001) and the relative risk was 2.48 and the Odds Ratio 4.89.
Conclusions:
In this study of Mexican population on infectious blepharitis and polymorphism of the promoter region of TLR 9 gene, we found high frequency for -1486T allele in patients with infectious blepharitis,which indicates that there is relationship between -1486T polymorphism in TLR 9 gene and is associated with the susceptibility for infectious blepharitis in the studied population.
Keywords: 480 cornea: basic science