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Liya Wang, Hongmin Zhang, Susu Liu, Jin Li, Hui Liu, Yanting Xie; The influence of non-steroidal anti-inflammatory drug on the effects of antifungal drug in treating experimental fungal keratitis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2826.
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© ARVO (1962-2015); The Authors (2016-present)
To observe the therapeutic effects of antifungal eye drop combined with diclofenac sodium (DF) eye drop on corneal fungal keratitis (FK).
C57 BL/6J mice were randomly grouped to wound group (WG), FK model group (MG), ketoconazole treatment group (KG) and diclofenac sodium (DF) treatment group (treated with ketoconazole and DF) (DFG). Each group was examined with slit lamp microscope and in vivo confocal scanning microscope at 1d, 2d, 3d, 4d and 5d after operation. The severity of corneal disease was evaluated by clinical score with slit lamp microscope. The hypha areas and the number of inflammatory cells in in vivo confocal microscopy images were quantitated. The corneal pathological examination of WG and DFG were conducted with H&E staining at 5d post-operation.
Clinical scores of cornea in MG, KG and DFG were significantly increased each time point than that in WG (P < 0.01). The clinical scores were significantly increased at 5d post-operation in DF G than that in the MG (P < 0.01). The clinical scores were significantly increased at 3d, 4d and 5d post-operation in DFG than that in KG (P < 0.01). The corneal hypha disappeared at 3d post-operation in MG and KG. The area of corneal hypha has no significant change in DFG compared with MG and KG (P > 0.05). The number of corneal inflammatory cells in MG, KG and DFG was significantly increased at each time point than that in the WG (P < 0.01). No significant differences of the number of inflammatory cells were seen in KG compared with MG (P > 0.05). The number of inflammatory cells in DFG was significantly increased within 4-5d than that in MG and KG (P < 0.01). The Pearson correlation analysis of corneal inflammatory cells and clinical scores in DFG showed that inflammatory cells infiltration and corneal disease severity was positively correlated (r = 0.86, P = 0.000). The HE staining showed that the cells in corneal stroma in WG were rare, and the cells in corneal stroma increased in DFG. In DFG, the cells in corneal stroma were 72.67% positive neutrophils ccording to differention count of stromal cells.
The results demonstrated that it should be cautious to use DF in treating FK. Because the clinical scores, infiltration of corneal inflammatory cells which is mainly neutrophils were significantly increased in using KT combined with DF in eye drop treating FK.
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