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Yongjie Qin, Kai On Chu, Yolanda Wong Ying Yip, Wai Ying Li, Sun On Chan, Chi Pui Pang; Green tea extract as a multiple compound is the most potent anti-inflammatory agents in endotoxin-induced uveitis.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2879.
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We have previously shown epigallocatechin-3-gallate (EGCG) is the most biologically active constituent of catechins in green tea extract (GTE). In this study we determine the anti-inflammatory effects of GTE, catechin mixtures and EGCG alone on endotoxin-induced uveitis (EIU) in Sprague Dawley (SD) rats.
EIU in rats was induced by a footpad injection of 1 mg/kg lipopolysaccharide (LPS), followed by 2 times oral administration of GTE (550 mg/kg), catechins mixtures, EGCG (375.2 mg/kg), and Dexamethasone (Dxm, 1 mg/kg) at 2 hours and 9 hours post-injection. Twenty four hours after injection, the eyes were examined by slit-lamp prior to terminating the animals for collection of aqueous humors for cell count and protein assay or enucleation of eyeballs for immunohistochemistry.
LPS caused severe hyperemia and edema in the iris, substantial accumulation of infiltrated cells and total protein in the aqueous humor. The infiltrated cells in anterior/posterior chambers and iris stroma were immunopositive for CD 43 (cell surface antigen on leukocytes) and CD68 (cytoplasmic antigen in monocytes/macrophages), with 80% of these cells being leukocytes and 20% monocytes/macrophages. GTE, catechin mixtures and EGCG all significantly reduced the infiltrated cell number and total protein concentration in aqueous humor (P<0.05). All animals treated with GTE (n=5 rats) and catechins mixtures (n=5 rats) survived after the treatment, but two of five rats died after EGCG treatment.
Our data showed that GTE and its catechins combination exhibited a potent anti-inflammatory action in an experimental model of acute ocular inflammation. Further experimentations are being done to investigate whether more potent therapeutic action is obtained at lower dosages and with different combinations of catechins.
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