April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Stress-Induced Damage to the Ocular Surface Resembling Dry Eye can Occur Independently of Chronic T cell-mediated Disease
Author Affiliations & Notes
  • Michael E Stern
    Biological Sciences, Allergan, Inc, Irvine, CA
    Ophthalomology, Baylor School of Medicine, Houston, TX
  • Katherine S Held
    Biological Sciences, Allergan, Inc, Irvine, CA
  • Chris S Schaumburg
    Biological Sciences, Allergan, Inc, Irvine, CA
  • Euikyon Oh
    Biological Sciences, Allergan, Inc, Irvine, CA
  • Sveti Ugarte
    Biological Sciences, Allergan, Inc, Irvine, CA
  • Larry A Wheeler
    Biological Sciences, Allergan, Inc, Irvine, CA
  • Margarita Calonge
    Ophthalmology, IOBA - University of Valladolid, Valladolid, Spain
  • Jerry Y Niederkorn
    Ophthalmology, UT Southwestern School of Medicine, Dallas, TX
  • Stephen C Pflugfelder
    Ophthalomology, Baylor School of Medicine, Houston, TX
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3059. doi:
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      Michael E Stern, Katherine S Held, Chris S Schaumburg, Euikyon Oh, Sveti Ugarte, Larry A Wheeler, Margarita Calonge, Jerry Y Niederkorn, Stephen C Pflugfelder; Stress-Induced Damage to the Ocular Surface Resembling Dry Eye can Occur Independently of Chronic T cell-mediated Disease. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3059.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: CD4+ T cells make a significant contribution to chronic inflammation during the immunopathogenesis of Dry Eye. To begin to understand the contribution of T cell-independent mechanisms of disease, T cell deficient RAG1-/- mice were exposed to desiccating stress (DS), and evaluated for abnormalities in corneal epithelial cells, proinflammatory cytokine expression and inflammatory cell infiltration over a 16 day exposure.

Methods: Dry Eye disease was induced by exposing female RAG1-/- mice to desiccating stress (DS: subcutaneous scopolamine, 0.1mg/day; humidity 20%; sustained airflow). Mice were evaluated for corneal fluorescein staining, and ocular surface tissues and tears were collected for analysis at various time points. Immunohistochemistry was used to evaluate neutrophil and monocyte infiltration at 0, 3, 6 and 16 days of DS.

Results: RAG1-/- mice exposed to DS displayed a significant increase (p≤0.001) in corneal fluorescein staining on Days 3, 6, 10 and 16 days DS. Immunohistochemistry revealed a significant (p≤0.001) increase in neutrophils in the conjunctiva at Day 6 (7.9±1.2), Day 10 (8.5±1.4) and Day 16 (10.2±2.1) in RAG1-/- mice exposed to DS compared to naïve mice (2.1±0.4). A similar trend was observed for increased CD11b+ cells in the conjunctiva at Day 6 (34.5±5.7; p≤0.05), Day 10 (40.3±4.5; p≤0.01) and Day 16 (46.3 ±3.7; p≤0.001) in DS mice relative to naïve mice (23.1±3.4). A significant (p≤0.05) increase in CD11b+ cells was also observed in the cornea at Day 16 (16.3±3.4) of DS compared to naïve mice (9.9±1.3).

Conclusions: These data demonstrate that stress-induced damage to the ocular surface resembling Dry Eye can occur independently of chronic T cell-mediated disease.

Keywords: 486 cornea: tears/tear film/dry eye • 557 inflammation  
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