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Vinay Aakalu, Sarmad Hassan Jassim, Amy Lin, Assraa H Jaboori, Pete Setabutr, Sandeep Jain; Accessory Lacrimal Gland (ALG) Gene Expression from Human Muller Muscle Conjunctival Resection (MMCR) Specimens Obtained via Laser Capture Microdissection (LCM). Invest. Ophthalmol. Vis. Sci. 2014;55(13):3115. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
MMCR specimens taken during routine ptosis surgery frequently contain ALG in addition to other tissue types. ALG gene expression patterns have been studied only very recently and the data available is minimal. To determine gene expression patterns of ALG isolated using LCM, including precursor cell genes, using PCR from MMCR specimens that contain multiple tissue types.
A Leica LCM was performed to isolate ALG from MMCR specimens. Following RNA extraction, the gene expression patterns of ALG, including precursor genes (ABCG2, CD133, CD49f, and CD90) was determined using PCR.
We determined a unique pattern of gene expression in ALG, which was compared with known expression patterns of other exocrine glandular tissue. LCM proved an efficient means of isolating ALG from MMCR specimens, which contain multiple cell and tissue types.
ALG cells can be isolated from MMCR specimens using LCM and a unique pattern of gene expression, including that of precursor genes is presented.
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