April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Expression of Angiotensin II and its role in human Tenon's capsule fibroblasts proliferation, migration and phenotype transition
Author Affiliations & Notes
  • Huimin Shi
    Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, China
  • Yiqin Xiao
    Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, China
  • Wen Ye
    Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, China
  • Footnotes
    Commercial Relationships Huimin Shi, None; Yiqin Xiao, None; Wen Ye, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3199. doi:
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      Huimin Shi, Yiqin Xiao, Wen Ye; Expression of Angiotensin II and its role in human Tenon's capsule fibroblasts proliferation, migration and phenotype transition. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3199.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To examine the expression of Angiotensin II (Ang II) and type I, type II receptors (AT1, AT2) in Tenon's capsule fibroblasts after rabbit trabeculectomy, and to investigate the role of Ang II in human Tenon's capsule fibroblasts (HTFs) proliferation, migration, phenotype transition and extracellular matrix (ECM) synthesis.

 
Methods
 

1. In Vivo: We performed trabeculectomy on rabbit eyes and used immunohistochemical staining to investigate protein expression of Ang II, AT1 and AT2 in Tenon's capsule fibroblasts. 2. In Vitro: HTFs were obtained from patients during cataract surgery and treated with increasing concentrations of Ang II from 10-10M to 10-5M, transforming growth factor β1 (TGF-β1) (10ng/mL) and vehicle as control. Cell proliferation was investigated by CCK-8 assay, migration by wound healing assay and transwell assay. Protein expression of alpha smooth muscle actin (α-SMA) and Collagen I (Col I) were measured by western blot and immunofluorescence. Statistical significance was assumed if P<0.05.

 
Results
 

In animal models, the expression of Ang II and AT1 increased from day 1 after surgery while AT2 increased from day 7. In cultured HTFs, Ang II promoted cell proliferation. The most robust cell proliferation was observed between concentrations of 10-9M and 10-7M of Ang II (P<0.05). In terms of cell migration and phenotype transition to myofibroblasts(MFs), low concentration of Ang II (10-8M) was more effective than high concentration (10-5M) (P<0.05). In addition, Ang II markedly induced α-SMA, protein expressed by MFs, and Col I expression. Stimulation with angiotensin II (between 10-9M and 10-7M) for around 2 to 3 days caused maximum protein expression.

 
Conclusions
 

Our results suggest that Ang II increases HTFs proliferation, migration and phenotype transition, causing wound healing after trabeculectomy in vivo and in vitro.

 
 
IHC-Expression of Ang II, AT1 and AT2 on Tenon's capsule fibroblasts of rabbits
 
IHC-Expression of Ang II, AT1 and AT2 on Tenon's capsule fibroblasts of rabbits
 
 
Cell Immunofluorescence-Expression of a-SMA on HTFs treated with Ang II
 
Cell Immunofluorescence-Expression of a-SMA on HTFs treated with Ang II
 
Keywords: 765 wound healing • 512 EMT (epithelial mesenchymal transition) • 474 conjunctiva  
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