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Shuhao Fu, Yiqin Xiao, Wen Ye; The up-regulation of the activin receptor-like kinase5 in the subconjunctival wound healing. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3208.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the change of the expression of activin receptor-like kinase5 (ALK5) in cell model of fibrosis and animal model of bleb scarring after filtration Surgery.
1. Fourteen New Zealand rabbits underwent filtration surgery on their right eye. Paraffin section of the eyes were labeled with HE staining, α-smooth muscle actin (α-SMA) and ALK5 were observed by immunohistochemical detection.2. Human tenon’s capsule fibroblast (HTF) was identificated and induced by 10ug/ul of TGF-β1. α-SMA and fibronectin (FN) was measured by Western blot to certify the existence of cell phenotype transformation and fibrosis. ALK5 mRNA level was quantified by real-time PCR, while Western blot and immunocytochemistry were used to detect the expression changes of ALK5 protein.
1.Immunohistochemistry showed that ALK5 was not obviously observed in subconjunctival tissue 3 days after operation. By day 7, immunoreactivity increased concomitant with increased cellularity of the wound. By day 14, Spindle-shaped fibroblasts, probably myofibroblasts were strongly immunoreactive around the wound. Till day 28, the immunoreactivity was reduced but still performed. 2. After TGF- β1 stimulation of HTFs , the protein expression of α-SMA and FN were up-regulated. ALK5 mRNA was up-regulated with a peak at 12 to 24 hours while protein expression was up-regulated with the peak around 24 to 36 hours.
The expression of ALK5 was up-regulated both in the cell model and animal model. It suggests that ALK5 may play an important role in the bleb scar formation after filtration surgery.
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