Purchase this article with an account.
Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae; Interleukin(IL)-33 and Thymic Stromal Lymphopoietin (TSLP), but not IL-25, induced Allergic Conjunctivitis in Mouse Models Papain-Induced Conjunctivitis.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3231.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Interleukin(IL)-33 as well as Thymic stromal lymphopoietin (TSLP) and IL-25 induce Th2 cytokine production by various types of cells, suspecting to involvement of these cytokines in development of allergic disorders. Supportively, we reported that IL-33 expression was increased in conjunctival epithelium of the giant papillae from patients with chronic allergic conjunctivitis (IOVS. 50:4646-52, 2009). In association with this, in the present study, we investigated the roles of IL-33 IL-25 and TSLP in papain-induced conjunctivitis using IL-33-deficient (IL-33-/-), IL-25-/- and TSLP receptor (TSLPR) -/- mice.
Papain-induced conjunctivitis in mice was established as below; C57BL/6 mice were had contact lenses (2mm diameter), which were soaked in papain solution for a right eye or in heat-inactivated papain solution as controls for a left eye. After fitting contact lenses, the eyelids were sutured. Two days later, the contact lenses were replaced by a new papain-soaked or control lens. Four days after first papain installation, histological analysis and gene expression analysis by realtime PCR were carried out in the resected conjunctival samples.
The number of eosinophils in conjunctiva and the expression levels of IL-4 and IL-13 mRNA were significantly reduced in IL-33-/- and TSLPR-/-, but not IL-25-/-, mice in comparison with wild-type mice during papain-induced conjunctivitis (IL-33-/- mice: 10.4 ± 5.2 P< 0.05, TSLPR-/- mice: 22.5 ± 4.5 P< 0.05, IL-25-/- mice: 32.9 ± 4.5 P> 0.05, versus wild-type mice: 40.6 ± 9.8, mean number of eosinophil ± SD per slides, n = 10, by Mann-Whitney’s U test).
IL-33 and TSLP, but not IL-25, produced by conjunctiva epithelial cells are crucial for the development of papain-induced allergic conjunctivitis. Our findings suggest that neutralization of IL-33 and TSLP may provide a potential target for novel therapeutic treatment for allergic conjunctivitis.
This PDF is available to Subscribers Only