April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
Ang-2 mediates processes of lymphatic formation
Author Affiliations & Notes
  • Sammy Grimaldo
    UC Berkeley, Berkeley, CA
  • Don Yuen
    UC Berkeley, Berkeley, CA
  • Lu Chen
    UC Berkeley, Berkeley, CA
  • Footnotes
    Commercial Relationships Sammy Grimaldo, None; Don Yuen, None; Lu Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3233. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Sammy Grimaldo, Don Yuen, Lu Chen; Ang-2 mediates processes of lymphatic formation. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3233.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction is associated with many disorders from cancer metastasis to transplant rejection. However, to date, there is still little effective treatment for lymphatic disorders. This study is to investigate the specific roles of Ang-2 in corneal lymphangiogenesis (LG) and lymphatic endothelial cell (LEC) functions in vitro.

Methods: Murine suture-induced corneal inflammatory LG and human primary LEC culture models were used in the study. The expressional changes of Ang-2 during corneal inflammation were assessed at both mRNA and protein levels. Additionally, human LECs were transfected with small interfering RNA (siRNA) against Ang-2 or nonspecific scramble control. The effects of Ang-2 gene knockdown on cell proliferation and tube formation were both analyzed.

Results: Ang-2 expression was significantly up-regulated in inflamed cornea. Ang-2 was expressed on both lymphatic vessels and macrophages. Moreover, Ang-2 gene knockdown in LECs suppressed both proliferation and tube formation of these cells.

Conclusions: These new findings indicate that Ang-2 is critically involved in lymphatic processes. Further investigation on this pathway may provide novel insights and therapeutic strategies for lymphatic disorders, which occur both inside and outside the eye.

Keywords: 609 neovascularization • 480 cornea: basic science • 479 cornea: clinical science  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.