April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
Alkali versus suture-induced corneal neovascularization: an overview of two common animal models of corneal neovascularization
Author Affiliations & Notes
  • Chiara Giacomini
    San Raffaele Scientific Inst, Milan, Italy
  • Giulio Ferrari
    San Raffaele Scientific Inst, Milan, Italy
  • Fabio Bignami
    San Raffaele Scientific Inst, Milan, Italy
  • Paolo Rama
    San Raffaele Scientific Inst, Milan, Italy
  • Footnotes
    Commercial Relationships Chiara Giacomini, None; Giulio Ferrari, None; Fabio Bignami, None; Paolo Rama, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3236. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Chiara Giacomini, Giulio Ferrari, Fabio Bignami, Paolo Rama; Alkali versus suture-induced corneal neovascularization: an overview of two common animal models of corneal neovascularization. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3236.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: To quantify and compare corneal hem- and lymphangiogenesis between alkali burn- and suture-induced corneal neovascularization (CNV) in C57BL/6 and FVB mouse strains.

Methods: A retrospective analysis was performed on 6 to 12 alkali burn- and suture-induced neovascularized corneas in 6- to 10-week old C57BL/6 and FVB mice. CNV was induced by (i) surface caustication with NaOH or (ii) intrastromal placement of three 10.0 nylon sutures. Four, 7, 10 and 14 days later mice were sacrificed and eyes collected for immunofluorescence analysis. Hemangiogenesis and lymphangiogenesis were quantitatively analyzed on whole mounted corneas using anti-CD31 and anti-LYVE1 antibodies. Data were analyzed by unpaired Student’s t-test and differences between groups were considerated statistically significant with P < 0.05.

Results: Blood vessel growth was similar between (i) alkali burn and suture-induced CNV in C57BL/6 mice, and (ii) between C57BL/6 and FVB sutured strains. On the contrary, corneal lymphangiogenesis was more pronounced in the C57BL/6 sutured mice versus the alkali burn group, and in the FVB strain versus both C57BL/6 models.

Conclusions: Significant differences occur in lymphangiogenesis, but not hemangiogenesis, in the alkali burn and suture-induced models in C57BL/6 mice. Furthermore, lymphangiogenesis is more pronounced in the albino (FVB) strain after suture placement. We suggest that the suture model has a number of advantages and may be preferentially used to study corneal lymphangiogenesis.

Keywords: 609 neovascularization • 480 cornea: basic science  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.