April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Conditional knockout of Wntless Suppresses Corneal Lymphangiogenesis
Don Yuen; Michael Wang; Eric Huang; Nilson Wu; Tatiana Ecoiffier; April Smith; Richard A Lang; Lu Chen
Author Affiliations & Notes
  • Don Yuen
    School of Optometry, Univ of California at Berkeley, Berkeley, CA
  • Michael Wang
    School of Optometry, Univ of California at Berkeley, Berkeley, CA
  • Eric Huang
    School of Optometry, Univ of California at Berkeley, Berkeley, CA
  • Nilson Wu
    School of Optometry, Univ of California at Berkeley, Berkeley, CA
  • Tatiana Ecoiffier
    School of Optometry, Univ of California at Berkeley, Berkeley, CA
  • April Smith
    Divisions of Ophthalmology and Developmental Biology, Cincinnati Children, Cincinnati, OH
  • Richard A Lang
    Divisions of Ophthalmology and Developmental Biology, Cincinnati Children, Cincinnati, OH
  • Lu Chen
    School of Optometry, Univ of California at Berkeley, Berkeley, CA
  • Footnotes
    Commercial Relationships Don Yuen, None; Michael Wang, None; Eric Huang, None; Nilson Wu, None; Tatiana Ecoiffier, None; April Smith, None; Richard Lang, None; Lu Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3241. doi:
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      Don Yuen, Michael Wang, Eric Huang, Nilson Wu, Tatiana Ecoiffier, April Smith, Richard A Lang, Lu Chen; Conditional knockout of Wntless Suppresses Corneal Lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3241.

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Abstract

Purpose: Corneal lymphangiogenesis accompanies many diseases after an inflammatory, infectious, immunogenic, or chemical insult. This study is to investigate whether Wnt-signaling pathway is involved in this pathological process using conditional knockout mice of Wntless (Wls), which binds to Wnt proteins and facilitates Wnt sorting and secretion.

Methods: Macrophage specific conditional knockout mice of Wls were generated by cross breeding of Csf1R-cre and Wls-flox mice. Suture placement model was used to induce corneal inflammatory lymphangiogenesis (LG). Digital images from immunofluorescent microscopic assays were analyzed by NIH Image J software.

Results: Inflammatory LG response in macrophage specific Wls conditional knockout mice was significantly suppressed, compared to control littermates, as assessed by invasion area.

Conclusions: This study provides the first evidence that macrophage derived Wnt-signaling is critically involved in inflammatory LG. Further investigation on this Wnt pathway may provide novel insights and strategies to treat lymphatic disorders, which occur both inside and outside the eye, such as transplant rejection, inflammation, and cancer metastasis.

Keywords: 609 neovascularization • 557 inflammation  
© 2014, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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