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Don Yuen, Michael Wang, Eric Huang, Nilson Wu, Tatiana Ecoiffier, April Smith, Richard A Lang, Lu Chen; Conditional knockout of Wntless Suppresses Corneal Lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3241.
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© ARVO (1962-2015); The Authors (2016-present)
Corneal lymphangiogenesis accompanies many diseases after an inflammatory, infectious, immunogenic, or chemical insult. This study is to investigate whether Wnt-signaling pathway is involved in this pathological process using conditional knockout mice of Wntless (Wls), which binds to Wnt proteins and facilitates Wnt sorting and secretion.
Macrophage specific conditional knockout mice of Wls were generated by cross breeding of Csf1R-cre and Wls-flox mice. Suture placement model was used to induce corneal inflammatory lymphangiogenesis (LG). Digital images from immunofluorescent microscopic assays were analyzed by NIH Image J software.
Inflammatory LG response in macrophage specific Wls conditional knockout mice was significantly suppressed, compared to control littermates, as assessed by invasion area.
This study provides the first evidence that macrophage derived Wnt-signaling is critically involved in inflammatory LG. Further investigation on this Wnt pathway may provide novel insights and strategies to treat lymphatic disorders, which occur both inside and outside the eye, such as transplant rejection, inflammation, and cancer metastasis.
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