April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
The effect of topical Epigallocatechin Gallate on corneal neovascularization in rabbits
Author Affiliations & Notes
  • Jae Yon Won
    Department of Ophthalmology and Visual Science, The Catholic University of Korea, Seoul, Republic of Korea
  • Chang Hyun Koh
    Department of Ophthalmology and Visual Science, The Catholic University of Korea, Seoul, Republic of Korea
  • Sung Kun Chung
    Department of Ophthalmology and Visual Science, The Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Jae Yon Won, None; Chang Hyun Koh, None; Sung Kun Chung, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3242. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jae Yon Won, Chang Hyun Koh, Sung Kun Chung; The effect of topical Epigallocatechin Gallate on corneal neovascularization in rabbits. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3242.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate the efficacy of topical application of epigallocatechin gallate(EGCG) for the treatment of corneal neovascularization in a rabbit model.

Methods: Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (24 eyes) for a week. After suturing, one randomly chosen eye of the 12 rabbits were treated with topical epigallocatechin gallate at two different concentrations: 0.01% (group 1) and 0.1% (group 2) while the contralateral eyes were treated with sterilized balanced salt solution as the control. All eye drops were applied twice daily for 2 weeks after suturing. The surface area of corneal neovascularization was measured and analyzed in all eyes on days 7 and 14. On day 14, all eyes were extracted to measure the expression of vascular endothelial growth factor (VEGF) mRNA and cyclooxygenase-2 (COX-2) protein in the corneal tissue.

Results: Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (24 eyes) for a week. After suturing, one randomly chosen eye of the 12 rabbits were treated with topical epigallocatechin gallate at two different concentrations: 0.01% (group 1) and 0.1% (group 2) while the contralateral eyes were treated with sterilized balanced salt solution as the control. All eye drops were applied twice daily for 2 weeks after suturing. The surface area of corneal neovascularization was measured and analyzed in all eyes on days 7 and 14. On day 14, all eyes were extracted to measure the expression of vascular endothelial growth factor (VEGF) mRNA and cyclooxygenase-2 (COX-2) protein in the corneal tissue.

Conclusions: Topical administration of EGCG effectively inhibits corneal neovascularization in rabbits. This inhibitory effect is probably related to the suppression of VEGF and COX-2 meditated angiogenesis.

Keywords: 480 cornea: basic science • 482 cornea: epithelium  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×