April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Tyrosine-mutated AAV2 mediated BDNF gene therapy attenuates retinal ischemic injuries in rats
Tsutomu Igarashi; Koichi Miyake; Maika Kobayashi; Kazuhisa Takahashi; Noriko Miyake; Osamu Iijima; Kenji Nakamoto; Yukihiko Hirai; Takashi Shimada; Hiroshi Takahashi
Author Affiliations & Notes
  • Tsutomu Igarashi
    Ophthalmology, Nippon Medical School, Tokyo, Japan
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Koichi Miyake
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Maika Kobayashi
    Ophthalmology, Nippon Medical School, Tokyo, Japan
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Kazuhisa Takahashi
    Ophthalmology, Nippon Medical School, Tokyo, Japan
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Noriko Miyake
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Osamu Iijima
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Kenji Nakamoto
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Yukihiko Hirai
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Takashi Shimada
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Hiroshi Takahashi
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Footnotes
    Commercial Relationships Tsutomu Igarashi, None; Koichi Miyake, None; Maika Kobayashi, None; Kazuhisa Takahashi, None; Noriko Miyake, None; Osamu Iijima, None; Kenji Nakamoto, None; Yukihiko Hirai, None; Takashi Shimada, None; Hiroshi Takahashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3320. doi:
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      Tsutomu Igarashi, Koichi Miyake, Maika Kobayashi, Kazuhisa Takahashi, Noriko Miyake, Osamu Iijima, Kenji Nakamoto, Yukihiko Hirai, Takashi Shimada, Hiroshi Takahashi; Tyrosine-mutated AAV2 mediated BDNF gene therapy attenuates retinal ischemic injuries in rats. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3320.

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Abstract

Purpose: To examine the ability of brain-derived neurotrophic factor (BDNF) using triple mutant adeno-associated viral (AAV) type 2 vector on retinal ischemia reperfusion injury.

Methods: Retinal ischemia was induced in rats by raising intraocular pressure (IOP) to 110 mm Hg two weeks after gene delivery. The neuroprotective effects of BDNF were evaluated by determining the preservation of the inner retina thickness and the cell counts in the cell counts in the retinal ganglion cell (RGC) layer one week after reperfusion. In addition, electroretinograms (ERGs) were performed to determine the functionality of the retina.

Results: Gene delivery significantly improved the recovery of retinal thickness (No treatment; 78µm ± 3.6, Control; 42µm ± 10.3, AAV-BDNF; 77µm ± 8.1) and rescued b-wave (No treatment; 975µV ± 191, Control; 396µV ± 231, AAV-BDNF; 882µV ± 155).

Conclusions: Triple mutant AAV type 2 vector mediated BDNF extremely protected rat retina from ischemia-reperfusion injury.

Keywords: 538 gene transfer/gene therapy • 615 neuroprotection  
© 2014, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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