April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Postmortem Ultrasound and OCT Imaging of the Posterior Segment
Author Affiliations & Notes
  • Peter Reed Pavan
    Eye Institute, University of South Florida, Tampa, FL
  • Mitchell D McCartney
    Ocular Research Center, Lions Eye Institute for Transplant & Research, Tampa, FL
  • Timothy Saunders
    Eye Institute, University of South Florida, Tampa, FL
  • Patrick Gore
    Ocular Research Center, Lions Eye Institute for Transplant & Research, Tampa, FL
  • Nicholas Sprehe
    Ocular Research Center, Lions Eye Institute for Transplant & Research, Tampa, FL
  • Wyatt Saxon
    Eye Institute, University of South Florida, Tampa, FL
  • Curtis E Margo
    Eye Institute, University of South Florida, Tampa, FL
  • Footnotes
    Commercial Relationships Peter Pavan, None; Mitchell McCartney, None; Timothy Saunders, None; Patrick Gore, None; Nicholas Sprehe, None; Wyatt Saxon, None; Curtis Margo, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3358. doi:https://doi.org/
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      Peter Reed Pavan, Mitchell D McCartney, Timothy Saunders, Patrick Gore, Nicholas Sprehe, Wyatt Saxon, Curtis E Margo; Postmortem Ultrasound and OCT Imaging of the Posterior Segment. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3358. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To correlate optical coherence tomography (OCT) imaging with high frequency ultrasound images of the posterior pole in postmortem non-preserved donor tissue eyes.

 
Methods
 

Postmortem eye bank globes were enucleated using standard techniques and transported to the laboratory in saline soaked gauze moist chamber on ice. The fresh eyes were treated within 12 hours post mortem with topical drops of 10% phenylephrine and 1% tropicamide, given in two rounds, three minutes apart. The globes were oriented and secured to a Styrofoam head with the corneas facing forward; balanced salt solution was injected with a 32 gauge needle into the vitreous cavity approximately 4 mm posterior to the limbus to achieve physiologic pressure as measured by palpation. OCT raster line scanning (Heidelberg, Heidelberg, Germany) (OCT) images of the macula were obtained. The eyes were then reoriented in the Styrofoam head so the posterior pole was facing forward. A high-frequency (40 mHz) ultrasound biomicroscopy (UBM) probe (Ellex, Adelaide, Australia) covered with a water filled ultrasound transducer cover (ClearScan®; ESI, Inc., Plymouth, MN) was placed over the back of the eye to obtain images of the macula and adjacent retina. The eyes were fixed using a solution of 10% neutral formalin.

 
Results
 

Similar to previous studies, the pharmacologic agents increased the pupil diameter an average of 1.87 mm. OCT imaging of the macula identified much of the anatomy appreciated in an in vivo scan, although postmortem retinal changes imposed some limitations. The UBM showed recognizable retinal landmarks in the posterior pole and correlated well with pathology seen on the OCT images such as epiretinal membranes causing macular puckering.

 
Conclusions
 

UBM successfully imaged fine retinal structures in postmortem eyes. This additional technique can be used to screen postmortem eyes for disease-specific conditions.

 
 
OCT image through the center of the macula showing epiretinal membrane with macular puckering
 
OCT image through the center of the macula showing epiretinal membrane with macular puckering
 
 
UBM image of same macula also shows the epiretinal membrane with macular puckering
 
UBM image of same macula also shows the epiretinal membrane with macular puckering
 
Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 472 comparative anatomy  
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