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Min Joung Lee, Ah Young Ko, Jung Hwa Ko, Mee Kum Kim, Won Ryang Wee, Sang In Khwarg, Ho-Kyung Choung, Joo Youn Oh; Effects of Mesenchymal Stem/Stromal Cells in T cell-mediated Dry Eye Model in Mice. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3656.
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To investigate the effects of mesenchymal stem/stromal cells (MSCs) in T cell-mediated dry eye model in mice that was induced by concanavalin A (ConA) into lacrimal glands.
Either phosphate buffered saline (PBS) or Con A (10mg/ml) was injected into lacrimal glands in mice. Immediately after ConA injection, balanced salt solution (BSS), 1X103 MSCs, or 1X105 MSCs were injected periorbitally near lacrimal glands. One week later, tear production was measured by phenol red thread test, and levels of interleukin (IL) -2 and interferon (IFN)-γ were evaluated in the lacrimal glands and the ocular surface using real time RT-PCR. Infiltration of inflammatory cells and damage to the lacrimal glands were evaluated by histopathology, and ocular surface goblet cell count was performed. Also, infiltration of immune cells in lacrimal glands was analyzed by immunohistochemistry and flow cytometry.
Con A injection induced T cell-mediated dry eye in mice by causing T cell infiltration, increasing pro-inflammatory cytokine expression. Also tear production was significantly decreased (p = 0.0006) and goblet cell count was reduced (p = 0.02). A Both 1X103 MSCs and 1X105 MSCs significantly improved tear production (vs. ConA+BSS group, p=0.001 and p=0.0006, respectively). Also, MSCs significantly decreased the levels of IL-2 and IFN-γ in the ocular surface and lacrimal glands. Similarly, the numbers of IFN-γ-expressing CD4+ cells in lacrimal glands were significantly lower in MSC-treated groups compared to Con A+BSS group (p = 0.008 for 1X103 MSCs, p = 0.004 for 1X105 MSCs).
Periorbital injection of MSCs reduce inflammation of the ocular surface and lacrimal glands, and improve tear production in Con A-induced dry eye model in mice.
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