April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effects of Environmental Enrichment on Tear Secretion in Mice
Author Affiliations & Notes
  • Kokoro Sano
    Keio University School of Medicine, Tokyo, Japan
  • Motoko Kawashima
    Keio University School of Medicine, Tokyo, Japan
  • Akiko Ito
    Keio University School of Medicine, Tokyo, Japan
  • Imada Toshihiro
    Keio University School of Medicine, Tokyo, Japan
  • Ryuji Hisamura
    Keio University School of Medicine, Tokyo, Japan
  • Shigeru Nakamura
    Keio University School of Medicine, Tokyo, Japan
  • Mitsuhiro Watanabe
    Health Science Laboratory, Keio Research Institute at Shonan-Fujisawa Campus, Keio University, Tokyo, Japan
  • Kazuo Tsubota
    Keio University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships Kokoro Sano, None; Motoko Kawashima, None; Akiko Ito, None; Imada Toshihiro, None; Ryuji Hisamura, None; Shigeru Nakamura, None; Mitsuhiro Watanabe, None; Kazuo Tsubota, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3660. doi:
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      Kokoro Sano, Motoko Kawashima, Akiko Ito, Imada Toshihiro, Ryuji Hisamura, Shigeru Nakamura, Mitsuhiro Watanabe, Kazuo Tsubota; Effects of Environmental Enrichment on Tear Secretion in Mice. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3660.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The present studies demonstrate that environmental enrichment increases neural factors in various organs including the brain. We investigated the effects of environmental enrichment on tear secretion in mice.

Methods: Male C57BL/6JJcl mice were exposed to an enriched environment (n=6) compared to mice living in a standard environment (n=6) for 4 weeks. After 4 weeks, we restrained the mice once as an acute stress test for 4 hours. Tear secretion was measured by using cotton threads (Zone Quick) for 30 seconds at once a week, pre- and post-acute stress. Body weight was also measured at the same point in time.

Results: The tear secretion in the enriched environment (EE) group was significantly higher (2.5±0.5mm) than in the standard environment (ST) group (1.7±0.5mm)(p<0.05) at 1 week. Also the body weight in the EE group was significantly larger (21.4±0.6g, 22.4±0.6g) than in ST group (20.3±1.0g, 21.5±0.8g)(p<0.05) at 2 weeks and 3 weeks. After the acute stress test, tear secretion was on a downward trend in only the ST group. The body weight significantly decreased in both EE and ST groups. But this tendency was remarkable in ST group (p<0.01) compared to the EE group (p<0.05).

Conclusions: The results of this study suggest that environmental enrichment may weaken deleterious effects by an acute stress on the body in mice.

Keywords: 576 lacrimal gland • 726 stress response  
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