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Saori Takashina, Kousuke Noda, Maho Amano, Tetsu Ohashi, Yoko Dong, Satoshi Kinoshita, Wataru Saito, Atsuhiro Kanda, Shin-Idhiro Nishimura, Susumu Ishida; Profile of N-Glycans in Human Vitreous. Invest. Ophthalmol. Vis. Sci. 2014;55(13):368. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Glycans are biopolymers bearing biological information and regarded as the third major class of cellular macromolecules, following nucleic acids and proteins. Glycosylation is an important process of post-translational modification that changes the functions of proteins, and it has recently been reported that serum profile of N-glycans is largely altered in patients with systemic diseases. However, little is known about the profiles of glycans in ocular tissues. In this study, we analyzed the profile of N-glycans in human vitreous fluid obtained from patients with non-proliferative vitreoretinal diseases.
A total of 20 vitreous samples and 20 plasma samples (10 males and 10 females, mean age 64.6±4.7 y/o) were collected from patients with epiretinal membrane (ERM, n=9) or idiopathic macular hole (MH, n=11) who underwent pars plana vitrectomy at Hokkaido University Hospital from 2010 to 2013. Profiles of N-glycans in the vitreous and plasma samples were analyzed using the novel technique, “glycoblotting method”.
Thirteen N-glycans were detected in all the vitreous samples. All the glycans were included in 32 N-glycans detected in plasma samples. The concentration of N-glycans in vitreous samples (132.3±29.1pmol/100μg protein) was significantly lower compared with those in plasma samples (505.7±16.9pmol/100μg protein, P<0.001). Predominant N-glycan [(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2] was common in both groups, but the most of the other N-glycans content ratios were different. There was no significant difference in concentration and composition of N-glycans in the vitreous between ERM and MH, or between males and females either.
Our data for the first time provide the information on profile of N-glycans in human vitreous humor.
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