April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Profile of N-Glycans in Human Vitreous
Author Affiliations & Notes
  • Saori Takashina
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Kousuke Noda
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Maho Amano
    Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan
  • Tetsu Ohashi
    Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan
  • Yoko Dong
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Satoshi Kinoshita
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Wataru Saito
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Atsuhiro Kanda
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Shin-Idhiro Nishimura
    Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan
  • Susumu Ishida
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Footnotes
    Commercial Relationships Saori Takashina, None; Kousuke Noda, None; Maho Amano, None; Tetsu Ohashi, None; Yoko Dong, None; Satoshi Kinoshita, None; Wataru Saito, None; Atsuhiro Kanda, None; Shin-Idhiro Nishimura, None; Susumu Ishida, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 368. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Saori Takashina, Kousuke Noda, Maho Amano, Tetsu Ohashi, Yoko Dong, Satoshi Kinoshita, Wataru Saito, Atsuhiro Kanda, Shin-Idhiro Nishimura, Susumu Ishida; Profile of N-Glycans in Human Vitreous. Invest. Ophthalmol. Vis. Sci. 2014;55(13):368. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Glycans are biopolymers bearing biological information and regarded as the third major class of cellular macromolecules, following nucleic acids and proteins. Glycosylation is an important process of post-translational modification that changes the functions of proteins, and it has recently been reported that serum profile of N-glycans is largely altered in patients with systemic diseases. However, little is known about the profiles of glycans in ocular tissues. In this study, we analyzed the profile of N-glycans in human vitreous fluid obtained from patients with non-proliferative vitreoretinal diseases.

Methods: A total of 20 vitreous samples and 20 plasma samples (10 males and 10 females, mean age 64.6±4.7 y/o) were collected from patients with epiretinal membrane (ERM, n=9) or idiopathic macular hole (MH, n=11) who underwent pars plana vitrectomy at Hokkaido University Hospital from 2010 to 2013. Profiles of N-glycans in the vitreous and plasma samples were analyzed using the novel technique, “glycoblotting method”.

Results: Thirteen N-glycans were detected in all the vitreous samples. All the glycans were included in 32 N-glycans detected in plasma samples. The concentration of N-glycans in vitreous samples (132.3±29.1pmol/100μg protein) was significantly lower compared with those in plasma samples (505.7±16.9pmol/100μg protein, P<0.001). Predominant N-glycan [(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2] was common in both groups, but the most of the other N-glycans content ratios were different. There was no significant difference in concentration and composition of N-glycans in the vitreous between ERM and MH, or between males and females either.

Conclusions: Our data for the first time provide the information on profile of N-glycans in human vitreous humor.

Keywords: 541 glycoconjugates/glycoproteins  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×